ECM Flashcards

1
Q

define ECM

A

network of macromolecules that occupy the extracellular space

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2
Q

what makes the ECM

A

connective tissue/cell

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3
Q

functions of ECM

A

protect
support
strength
signal

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4
Q

what does the ECM store

A

growth factors

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5
Q

what is the ECM used for

A

cell adhesion/migration

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6
Q

what occurs with malignant cells

A

continue to grow without ECM or connection with other cells

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7
Q

what happens when cells do not have ECM

A

programmed cell death by anoikis

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8
Q

ECM proteins 6

A
collagen
elastin
fibronectin
laminin
proteoglycans
minerals
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9
Q

how is elastin secreted

A

soluble formed linked by disulphide bridges

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10
Q

what is fibronectin

A

large protein or 2 almsot identical protein (dimer)

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11
Q

functions of fibronectin

A

support and strength

interactions with other ECM proteins

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12
Q

fucntio of laminin

A

binding site for cell/protiens

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13
Q

what is laminin a component of

A

basal lamina

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14
Q

what is the basal lamina

A

layer of the basement membrane

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15
Q

what are proteoglycans

A

protein backbone with polysaccharide side chains

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16
Q

what do proteoglycans do

A

regulate protein activity and act as co receptors

17
Q

what are the side chains of proteoglycans called

A

glycosaminoglycans

18
Q

what is the basement membrane

A

network of macromolecules

specilaised form of ECM

19
Q

how do cells interact with the ECM

A

through integrins

20
Q

what are integrins

A

membrane bound receptros

21
Q

what are integrins made of

A

2 different protiens (heterodimer)

22
Q

what determins ligand binding

A

specific dimer pairing

23
Q

purpose of integrins 2

A

cells can detect what matrix they are bound to

generates a signal once bound

24
Q

how do cells modify the ECM

A

secrete proteilytic enzymes to break down ECM allowing path for cell migration

25
Q

what acts as a signal after ECM has been broken down

A

degradation productis

26
Q

what can enzymes do in responce to degradation products

A

secrete/activate GF

27
Q

What occurs at wound heeling

A

matrix signals
haemostat responce to prevent bleeding
cells move and proliferate at wound

28
Q

what is different in canner

A

matrix and integrin expression

29
Q

what does integrin signal for

A

growth
apoptosis
migration

30
Q

what is connective tissue called in cancer

A

stroma

31
Q

what happens during tutor metastasis

A

disregulated growth, decreased cell contact
basement membrane breaks down
timor cells invade stroma
regrow with angiogenesis

32
Q

how can the ECM vary

A

due to different composition

33
Q

what are elastic fibres arrangement and components

A

cross linked arrays of tropoelastin

34
Q

what is laminin

A

large protein of 3 different subunits

35
Q

function of laminin

A

binding site for cells/protein

36
Q

what does laminin signal cells to do

A

differentiate
adhere
migrate

37
Q

what can integrins also act as

A

mechanosensors