collagen

Question 1

what are the main groups of collagens

Answer 1

Fibril forming
FACIT
Network forming 
Microfibrillar 
Anchoring 
Transmembrane
Endo statin producing

Question 2

describe collagen synthesis

Answer 2

gene transcribed in nucleus
mRNA translated ribosomes
Alpha chains undergo post translational modification (Golgi)
chains wrap to for triple helix - tropocollagen
protein packaged into vehicles to outside of cell

Question 3

describe post translational modification of collagen on the golgi

Answer 3

hydroxylation of proline and lysine groups (for crosslinking)
glucose and galactosyl are attached

Question 4

what is needed for hydroxylation of proline an lysine

Answer 4

enzyme needs vit C

Question 5

describe collagen fibril formaiton

Answer 5

spontaneous
N-propeptides and C-propetides are cleaved by proteases
collagen monomers align into quarter staggered arrangement
molecular arrangement stabilised by formation of covalent cross link by lysyloxdase

Question 6

what heritable disorders affect genes of collagen

Answer 6

osteogenesis imperfecta

erlos-danlos syndrome

Question 7

deserve other diseases (not heritable)

Answer 7

vit C deficiency
inflammatory disease
autoimmune diseases

Question 8

role of FACIT collagens in fibril formation

Answer 8

control fibre diameter
spacer functions
ensures certain no fibres in fibre

Question 9

fibril forming collagen type I

Answer 9

bone
teeth
skin

Question 10

Type II fibril forming

Answer 10

cartilage

vitreous body

Question 11

Type III fibril forming

Answer 11

skin
Reticular fibres
Vessel walls

Question 12

Type V fibril forming

Answer 12

lung
cornea
bone

Question 13

Type XI fibril forming

Answer 13

cartilage virtreous body

Question 14

collagen I structure

Answer 14

trimeric
triple helix no interruptions
form fibrils

Question 15

describe collagen

Answer 15

3 polypeptide chains for form triple helical domains

Gly-y-x (proline and 4 hydroxy proline)

Question 16

what is glycine used for

Answer 16

helix turn

Question 17

what do network forming collagens do plus which no

Answer 17

form interlaced networks in basement membranes

4

Question 18

what do micro fibrillar collagens do and no

Answer 18

packing role

6

Question 19

what do anchoring fibrils do and no

Answer 19

anchor cells together

7

Question 20

what do transmembrane collagens do and no

Answer 20

communication between inside and outside

8

Question 21

what do end-stain producing collagens do and no

Answer 21

produce endo-stain once cleaned proteolytically

15

Question 22

functions of collagen

Answer 22

mediates cell signalling
storage of GF
adhesion, differentiation and cell activity