Canine Breeding Management Flashcards
Ovulation:
LH peaks how long into estrus? Caused by…..
24-48 hours.
estrogen peak
Ovulation:
LH has a very (short/long) peak duration?
very short. Assayed daily to detect the peak rise
Ovulation takes place how long after LH surge? What day of estrus is this around?
24-48 hours post LH surge
day 3-4 of estrus.
In the bitch, a _______ is ovuated (__N)m and must go through further division to a ______ (__N) before it is ready for fertilzation.
primary oocyte (2N)
Secondary oocyte (1N)
How long does it take a primary oocyte to become a secondary oocyte post ovulation?
How long is the secondary oocyte viable for fertilization?
2-3 days
3-4 days
What hormone is used as the modern standard for breeding management?
Progesterone - P4
Progesterone:
Semiquantitative or Quantitative methods preferred?
Quantitative
What are the two quantitative methods for progesterone measurement:
1.
2.
- Radioummunoassay - RIA
2. Chemoluminescence
Sampling schedule for Progesterone?
M-W-F
Inital rise in P4 coincides to _____
LH Peak
Start monitoring P4 3-4 days after ____ or when……
proestrus or when vaginal cornification is 50% superifical and 20% anucleated cells
Progesterone Evaluation: Values
- LH peak = ___ng/mL
- Ovulation starting = ___ng/mL
- Ovulation confirmed if > ___ng/mL
- 2 ng/mL
- 5 ng/mL
- 15 ng/mL
Failure to confirm ovulation is a common mistake that can be due to:
1.
2.
3.
- Conception failure
- Small litter (single pup syndrome)
- Inaccurate due date determination
When do you want to breed with natural or shipped semen after you have confirmed ovulation via P4 testing?
48 hr and 96 hrs after
When do you want to breed with frozen semen after you have confirmed ovulation via P4 testing?
72 hours
Embryos remain in oviduct for how many days after ovulation?
They enter the uterus as a _____ or _____
9-10 days
late morula or early blastocyst
Canine oocytes are good for up to ____hrs post-ovulation
108
Describe alternate day breeding for bitch:
breeding every 2-3 days until bitch no longer stands
Reasons to attempt alternate day breeding:
1.
3.
- Canine spermatozoa may be good for up to 6 days in genital tract of bitch
- Higher conception rates
- Later litter sizes
LH Assay:
A more or less definitive method than progesterone?
More definitive
Disadvantage of LH assays:
Must take daily blood samples
T/F: An animal with high LH has always just ovulated
F, spayed animals will have high LH due to no negative feedback
You want your male to have how many progressively motile morphologically normal spermatozoa for breeding?
200 * 10^6
Would like your male to have.
- ___% normal spermatozoa morphology?
- ___% normal motility?
- Should test negative for ____
- 80%
- 80%
- Brucella canis
