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Therio - RUSS > Canine Breeding Management > Flashcards

Canine Breeding Management Flashcards

1
Q

Ovulation:

LH peaks how long into estrus? Caused by…..

A

24-48 hours.

estrogen peak

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2
Q

Ovulation:

LH has a very (short/long) peak duration?

A

very short. Assayed daily to detect the peak rise

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3
Q

Ovulation takes place how long after LH surge? What day of estrus is this around?

A

24-48 hours post LH surge

day 3-4 of estrus.

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4
Q

In the bitch, a _______ is ovuated (__N)m and must go through further division to a ______ (__N) before it is ready for fertilzation.

A

primary oocyte (2N)

Secondary oocyte (1N)

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5
Q

How long does it take a primary oocyte to become a secondary oocyte post ovulation?

How long is the secondary oocyte viable for fertilization?

A

2-3 days

3-4 days

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6
Q

What hormone is used as the modern standard for breeding management?

A

Progesterone - P4

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7
Q

Progesterone:

Semiquantitative or Quantitative methods preferred?

A

Quantitative

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8
Q

What are the two quantitative methods for progesterone measurement:

1.
2.

A
  1. Radioummunoassay - RIA

2. Chemoluminescence

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9
Q

Sampling schedule for Progesterone?

A

M-W-F

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10
Q

Inital rise in P4 coincides to _____

A

LH Peak

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11
Q

Start monitoring P4 3-4 days after ____ or when……

A

proestrus or when vaginal cornification is 50% superifical and 20% anucleated cells

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12
Q

Progesterone Evaluation: Values

  1. LH peak = ___ng/mL
  2. Ovulation starting = ___ng/mL
  3. Ovulation confirmed if > ___ng/mL
A
  1. 2 ng/mL
  2. 5 ng/mL
  3. 15 ng/mL
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13
Q

Failure to confirm ovulation is a common mistake that can be due to:

1.
2.
3.

A
  1. Conception failure
  2. Small litter (single pup syndrome)
  3. Inaccurate due date determination
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14
Q

When do you want to breed with natural or shipped semen after you have confirmed ovulation via P4 testing?

A

48 hr and 96 hrs after

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15
Q

When do you want to breed with frozen semen after you have confirmed ovulation via P4 testing?

A

72 hours

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16
Q

Embryos remain in oviduct for how many days after ovulation?

They enter the uterus as a _____ or _____

A

9-10 days

late morula or early blastocyst

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17
Q

Canine oocytes are good for up to ____hrs post-ovulation

A

108

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18
Q

Describe alternate day breeding for bitch:

A

breeding every 2-3 days until bitch no longer stands

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19
Q

Reasons to attempt alternate day breeding:

1.

3.

A
  1. Canine spermatozoa may be good for up to 6 days in genital tract of bitch
  2. Higher conception rates
  3. Later litter sizes
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20
Q

LH Assay:

A more or less definitive method than progesterone?

A

More definitive

21
Q

Disadvantage of LH assays:

A

Must take daily blood samples

22
Q

T/F: An animal with high LH has always just ovulated

A

F, spayed animals will have high LH due to no negative feedback

23
Q

You want your male to have how many progressively motile morphologically normal spermatozoa for breeding?

A

200 * 10^6

24
Q

Would like your male to have.

  1. ___% normal spermatozoa morphology?
  2. ___% normal motility?
  3. Should test negative for ____
A
  1. 80%
  2. 80%
  3. Brucella canis
25
Q

Formula for normal size estimate of testes:

A

Scrotal Width = .324 ( log of body weight) + 1.249

26
Q

When assesing scrotum, be sure to assess for _____.

A

Symmetry

27
Q

Avoid including the ____ in testicular measurements

A

epididymis

28
Q

Most commonly used method of semen collection?

A

Manual stimulation

29
Q

Semen collection options:

  1. _____ used in conjunction with manual stimulation. Benefit?
  2. _____. Requires…..
A
  1. Chemical ejaculation. May increase spermatozoa numbers

2. Electroejaculation. Requires GA

30
Q

Manual collection of Semen:

  1. Hold hand caudal to ____, than slide prepuce over it.
A

bulbous glandis.

31
Q

3 Fractions of Manual Semen collection:

A

Pre-sperm, sperm-rich, prostatic portion

32
Q

Manual collection gets you how much sperm per ejaculate?

A

300 mil - 2 bil

33
Q

Options for Chemical Ejaculation:

1.
2.

A
  1. Oxytocin

2. Prostaglandin

34
Q

Chemical Ejaculation Techniques:

  1. Oxytocin?
  2. Prostaglandin?
A
  1. 5 IU subcuticular or IM 5-10 min prior to manual stimulation
  2. .1 mg/kg SubQ or IM 15 min prior to manual stimulation
35
Q

Semen Evaluation:

  1. Conc should be…
A

4-400 * 10^6/mL

36
Q

Semen Eval:

total sperm/ejaculation?

A

300-2000 * 10^6

37
Q

When performing trans-cervical insemination, the catheter may advance to the ____ which lies _______ to the cervial opening.

A

fornix

cranio-ventral

38
Q

Cooled Semen for AI:

  1. Avoid accidentally inserting catheter into ____
A

bladder

39
Q

Cooled Semen for AI:

Extend semen 1:1 to 1:4 with ____. Why?

A

commercial extender

remove excess fluids

40
Q

Semen Freezing:

Usually frozen in what forms?

A

on dry ice in pellets or straws

41
Q

Semen Freezing:

Avg dog may have __-__ pellets per dose, and should get about ___ doses per ejaculate.

A

10-12

3+

42
Q

Use of Frozen Semen:

Ned to breed using _____ or _____ method to ensure semen in uterus

A

Transcervical or Surgical

43
Q

Describe the surgical method of frozen semen insemination

A

midline laparotomy, use 22 g needle placed obliquely into lumen. Be sure to visualize distension of uterine horn with injection to ensure you are in lumen.

44
Q

Use (rigid/flexible) endoscope to visualize cervix during surgical insemination?

A

rigid

45
Q

Reasons why breeding won’t occur:

1.
2.
3.
4.
5.
6.
7.
A
  1. Female is in proestrus
  2. Vaginal anomaly - band of tissue
  3. Arthritis in male = pain when breeding
  4. Prostatic pain in male
  5. Penile issue
  6. Breed
  7. Uncooperative dogs
46
Q

Vaginal Strictures/Band:

Tend to be around what area?

A

Vestibulo-vaginal area

47
Q

Superfecundation =

A

Two males breed the same female during a single estrus.

48
Q

Superfetation =

A

where a female may be bed and get pregnant, and than go through another receptive period and get pregnant again - fetuses are different ages

not in dogs

Therio - RUSS (23 decks)

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