Bordatella pertusis and Pseudomanas

Question 1

name toxins for bordetella pertussis

Answer 1

pertussis toxin 
adenylate cyclase 
tracheal toxin 
FHA
dermonecrotic toxin
LPS lipid A and Lipid X

Question 2

which one of the bordetella pertussis toxin is lethal

Answer 2

dermonecrotic toxin

Question 3

what does the pertussis toxin catalyze? what does it result in

Answer 3

S1 subunit catalyses ADPR reaction of Gi
activate ACase
increase cAMP

Question 4

what role does ACase play in pertussis toxin

Answer 4

converts ATP to cAMP

Question 5

name 3 stages of pertussis

Answer 5

catarrhal
paroxysmal
convalescent

Question 6

what tests are used to detect pertussis

Answer 6

direct fluorescent antibody test

polymerase chain reaction

Question 7

what are the advantages of direct fluorescent antibody test

Answer 7

fast

Question 8

what are disadvantages of direct fluorescent antibody test

Answer 8

may not be specific

Question 9

what vaccine is used to treat pertussis

Answer 9

acellular Pertussis antigen vaccine

Question 10

what antibiotic is used for pertussis? what is a disadvantage

Answer 10

erythromycin: can’t treat if bacteria is already circulating body

Question 11

what is the main virulence factor for pseudomonas aeruginosa

Answer 11

bacterial biofilms

Question 12

why is pseudomonas aeruginosa a major cause of chronic infection

Answer 12

100-1000 fold more resistant to antibiotics

Question 13

where are the biofilms located

Answer 13

ubiquitous

Question 14

exotoxin A has a similar mode of action as

Answer 14

diphtheria toxin-ADP-ribosylation of EFII ( elongation factor II)

Question 15

exoenzyme S

Answer 15

required for dissemination from burn wounds and for tissue destruction in chronic lung infections

Question 16

Elastase

Answer 16

breaks down elastin, collagen, complement components

Question 17

what is “quorum sensing”

Answer 17

LasR (transcriptional activator) senses presence of Psudomonas

Question 18

what controls elastase production

Answer 18

LasR

Question 19

what regulates quorum sensing

Answer 19

Iron-regulated

Question 20

what does phohspholipase C and heat-stable phospholipase do

Answer 20

hydrolysis of phospholipids leading to tissue damage

Question 21

what does alkaline phosphotase do

Answer 21

proteolysis leading to tissue damage

Question 22

alginate

Answer 22

promotes adherence to respiratory epithelium, interferes with phagocytosis

Question 23

what type of infection is caused by pseudomonas

Answer 23

septicemia
meningitis
endocarditis
panophthalmitis

Question 24

panophthalmitis

Answer 24

inflammation of all coats of the eye including intraocular structures.

Question 25

what can cause pseudomonas falliculitis

Answer 25

immersion in contaminated water such as hot tubs

Question 26

when can pseudomonas folliculitis be a secondary infection

Answer 26

acne

epilate their legs

Question 27

what does x-ray of CF chest look like

Answer 27

bilateral bronchopneumonia

Question 28

contact lenses and pseduomonas infection

Answer 28

hemolysin

difficult to treat

Question 29

what type of therapy is used for pseudomonas

Answer 29

combination therapy: penicillin family and aminoglycoside

Question 30

cystic fibroses is located on what gene

Answer 30

chromosome 7

Question 31

what organs are impacted in CF

Answer 31

lungs and exocrine glands

Question 32

what type of mutation is CF

Answer 32

three nucleotide deletion

Question 33

name 3 pathogens that can colonize the CF lung

Answer 33

S aurerus
H influenzae
P. aeruginosa

Question 34

what is clinical significance for CF

Answer 34

• excessive neutrophil infiltration in lung
• infections around bronchi
• malnutrition