Bordatella pertusis and Pseudomanas Flashcards
name toxins for bordetella pertussis
pertussis toxin adenylate cyclase tracheal toxin FHA dermonecrotic toxin LPS lipid A and Lipid X
which one of the bordetella pertussis toxin is lethal
dermonecrotic toxin
what does the pertussis toxin catalyze? what does it result in
S1 subunit catalyses ADPR reaction of Gi
activate ACase
increase cAMP
what role does ACase play in pertussis toxin
converts ATP to cAMP
name 3 stages of pertussis
catarrhal
paroxysmal
convalescent
what tests are used to detect pertussis
direct fluorescent antibody test
polymerase chain reaction
what are the advantages of direct fluorescent antibody test
fast
what are disadvantages of direct fluorescent antibody test
may not be specific
what vaccine is used to treat pertussis
acellular Pertussis antigen vaccine
what antibiotic is used for pertussis? what is a disadvantage
erythromycin: can’t treat if bacteria is already circulating body
what is the main virulence factor for pseudomonas aeruginosa
bacterial biofilms
why is pseudomonas aeruginosa a major cause of chronic infection
100-1000 fold more resistant to antibiotics
where are the biofilms located
ubiquitous
exotoxin A has a similar mode of action as
diphtheria toxin-ADP-ribosylation of EFII ( elongation factor II)
exoenzyme S
required for dissemination from burn wounds and for tissue destruction in chronic lung infections
Elastase
breaks down elastin, collagen, complement components
what is “quorum sensing”
LasR (transcriptional activator) senses presence of Psudomonas
what controls elastase production
LasR
what regulates quorum sensing
Iron-regulated
what does phohspholipase C and heat-stable phospholipase do
hydrolysis of phospholipids leading to tissue damage
what does alkaline phosphotase do
proteolysis leading to tissue damage
alginate
promotes adherence to respiratory epithelium, interferes with phagocytosis
what type of infection is caused by pseudomonas
septicemia
meningitis
endocarditis
panophthalmitis
panophthalmitis
inflammation of all coats of the eye including intraocular structures.
what can cause pseudomonas falliculitis
immersion in contaminated water such as hot tubs
when can pseudomonas folliculitis be a secondary infection
acne
epilate their legs
what does x-ray of CF chest look like
bilateral bronchopneumonia
contact lenses and pseduomonas infection
hemolysin
difficult to treat
what type of therapy is used for pseudomonas
combination therapy: penicillin family and aminoglycoside
cystic fibroses is located on what gene
chromosome 7
what organs are impacted in CF
lungs and exocrine glands
what type of mutation is CF
three nucleotide deletion
name 3 pathogens that can colonize the CF lung
S aurerus
H influenzae
P. aeruginosa
what is clinical significance for CF
- excessive neutrophil infiltration in lung
- infections around bronchi
- malnutrition
