Anatomic Techniques Midterm Flashcards
the anhydrides DDSA, NMA, and NSA are used when preparing embedding media for tissues that will be studied with the
transmission electron microscope
The technique of autoradiography is based on
attachment of silver grains to specific cellular molecules
The method most commonly utilized when preparing tissues for study with the TEM is
primary fixation in glutaraldehyde followed by secondary fixation in osmium tetroxide
A scientist wishing to visualize primary cilia on the surface of a specific organ would need to
utilize a scanning electron microscope
One of the most common used fluorophores in immunofluorescent microscopy is
rhodamine
In immunofluorescence microscopy how many antibodies are required for tissue processing?
2
What are the commonalities b/w confocal & immunofluorescence microscopy
light passes through or is reflected from the sample
In confocal microscopy, the following is used to block out of focus light
pin hole
Confocal and immunofluorescence microscopy are considered with what type of microscopy?
optical microscopy
FRET
energy is transfered from a donor and to an acceptor with absorption of photons
What is a primary condition for fret to occur?
donor and acceptor molecules must be close in proximity, 1-10 nm
One of the main advantages of using FRET and bret assays over traditional biochemical assays is
protein-protein interaction can be determined in live cells
In BRET
no external light source & bioluminescence intensity is low, causing no fluorophore photo bleaching
The IVIS small animal imager
is not only used in oncology, but in many fields
To evaluate the movement of live cells
spinning disk microscopy would be best b/c its quicker and provides good resolution
