Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Repro > 6. Chlamydia diagnosis & treatment > Flashcards

6. Chlamydia diagnosis & treatment Flashcards

1
Q

how do you diagnose chlamydia? (lab techniques)

A

antigen detection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what are the different types of antigen detection carried out for testing chlamydia?

A 
immunofluorescence
enzyme immunoassays (EIA)
molecular methods (PCR)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

how do you carry out immunofluorescence in antigen detection carried out for testing chlamydia?

A

specimens may be fixed to a slide, and

stained with a monoclonal antibody that is tagged with fluorescein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

how are slides examined in immunofluorescence in antigen detection carried out for testing chlamydia?

A

examined under an UV microscpe

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what are the drawbacks of immunofluorescence in antigen detection carried out for testing chlamydia?

A

results are subject to observer error and the method is time consuming and therefore only suitable for small numbers of specimens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what is an advantage of immunofluorescence in antigen detection carried out for testing chlamydia?

A

quality of specimen in terms of cells can be assessed using this technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what does enzyme immunoassays (EIA) allow for in antigen detection carried out for testing chlamydia?

A

allows large numbers of specimens to be processed with relative ease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

advantages of enzyme immunoassays (EIA) allow for in antigen detection carried out for testing chlamydia?

A

some kits have good sensitivity / specificity

the tests are relatively cheap

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

disadvantages of enzyme immunoassays (EIA) allow for in antigen detection carried out for testing chlamydia?

A

commercial kits may vary in their sensitivity and specificity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what do molecular methods (PCR) offer in antigen detection carried out for testing chlamydia?

A

high sensitivity and specificity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what are the disadvantages of molecular methods (PCR) in antigen detection carried out for testing chlamydia?

A

clinical specimens may contain inhibitors that will interfere with the assay
commercial kits may yield significant false positives and negatives

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

what does Chlamydia trachomatis contain relatively little of?

A

peptidoglycan

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what is peptidoglycan the target for?

A

ß lactam antibiotics

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what is the mainstay of treatment for Chlamydia trachomatis?

A

macrolides

tetracyclines

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what are examples of macrolides?

A

erythromycin / clarithromycin / azithromycin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

how do macrolides and tetracyclines work?

A

inhibit protein synthesis

17
Q

how should conjunctivitis be treated? why?

A

treat with systemic antibiotics

widespread infection

Repro (78 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions