48. Enzymes Flashcards
protein catalysts that inc the velocity of chemical rxn and not consumed during the rxn
enzymes
are physically distinct versions of a given enzyme, each of which catalyzes the same rxn
isoenzymes
classes of enzymes
oxidoreductases transferases hydrolases lyases isomerase ligases
catalyze oxidations and reductions
Oxidoreductases
catalyze transfer of moieties such as glycosyl, ethyl or phosphoryl groups
transferases
catalyzed hydrolytic cleavage of C–C, C–O, C–N and other bonds
hydrolases
catalyze cleavage of C–C, C–O, C–N
and by other bonds by atom elimination , leaving double bonds
lyases
catalyze geometric or structural changes withi a molecule
isomerases
catalyze the joining together 2 molecules coupled with hydrolysis of ATP
ligases
will inc rate of rxn
positive effector
describes how rxn velocity varies with substrate conc
michealis menten eqn
enzymes that follow michealis menten kinetics have this curve
hyperbolic
max number of substrate molecules converted per unit time
maximal velocity
the substrate conc at which V1 is half the maximal velocity attainable at a particular conc of enyme
michealis constant (Km)
factors that affect the rate of rxn
substrate conc
temperature
pH
