1. Biotechnology

Question 1

is the production of recombinant DNA molecule that is self-perpetuating

Answer 1

cloning

Question 2

the strategy is to clone a gene into a vector that will readily be taken up and incorporated into the genome of a host

Answer 2

Gene therapy

Question 3

disease that may be tx with gene therapy

Answer 3

adenosine deaminase deficiency

chronic granulomatous disease

hemophilia

Question 4

probe binds to specific gene site of interest

specific localization of genes and direct visualization of anomalies at molecular level

Answer 4

Fluorescence in situ hybridization

Question 5

rapid immunologic technique testing for antigen-antbody reactivity

Answer 5

ELISA

Question 6

disease that may be diagnosed with FISH

Answer 6

Down syndrome (Trisomy 21)
Cat cry syndrome (5p deletion)
Chronic myelogenous leukemia (translocation 9:22)

Question 7

thousands of nuclei acid sequences are arranged in grids on glass and silicon

DNA or RNA probes are hybdridized to the chip and a scanner detects the relative amounts of complementary binding

measures many mRNA levels at once and detecs genomic changes

Answer 7

microarrays

Question 8

southern blot

sample
probe

Answer 8

sample : DNA

probe: cDNA

Question 9

northern blot

sample
probe

Answer 9

sample : RNA

probe: cDNA

Question 10

western blot

sample
probe

Answer 10

sample : protein

probe: antibody

Question 11

used to determine which restriction fragments of DNA are associated with a particular gene

Answer 11

Southern blot

Question 12

molecular biology lab procedure that is used to synthesize many copies of desired fragment DNA

Answer 12

PCR

Question 13

used to deduce original sequence of DNA

Dideoxynucleotides halt DNA polymerization at each base generating sequences of various lengths that encompass the entire sequence

Answer 13

sanger DNA sequencing