36. Special Stains/Cytochemistry

Question 1

1. A 66 year old woman presents with an LAP score of 8. What is a possible clinical correlation for this score ?

a.Normal
b.Leukemoid reaction
c.Chronic granulocytic leukemia
d.Pregnancy

Answer 1

c.Chronic granulocytic leukemi

Question 2

2. a 57 year old woman has an LAP score of 52. what is a possible clinical correlation for this score ?

a.Normal
b.Leukemoid reaction
c.Chronic granulocytic leukemia
d.Pregnancy

Answer 2

a.Normal

Question 3

3. In which condition would the majority of cells stain positive with myeloperoxidase and sudan black B ?

a.Acute lymphocytic leukemia
b.Acute myeloid leukemia
c.Chronic lymphocytic leukemia
d.Erythroleukemia

Answer 3

b.Acute myeloid leukemia

Question 4

4. Which of the following cells would demonstrate positive staining with Sudan black B?
   a.Lymphocyte
   b.Basophil
   c.Promyelocyte
   d.Erythroblast

Answer 4

c.Promyelocyte

Question 5

5.Which of the following cells would stain positive using the specific esterase stain?
a.Megakaryocytes
b.Macrophages
c.Neutrophils
d.Lymphocytes

Answer 5

c.Neutrophils

Question 6

6.Staining of which of the following cell lines would be inhibited by the addition of sodium fluoride to nonspe-cific esterase methods?
a.Megakaryocytes
b.Plasma cell
c. Lymphocyte
d.Monocyte

Answer 6

d.Monocyte

Question 7

7.For which of the following disease states would the acid phosphatase/TRAP stain have diagnostic value?
a.Erythroleukemia
b.Hairy-cell leukemia
c. Chronic lymphocytic leukemia
d. Burkitt lymphoma

Answer 7

b.Hairy-cell leukemia

Question 8

8.For which of the following disease states would periodic acid-Schiff staining have diagnostic value?
a.Erythroleukemia
b.Hairy-cell leukemia
c. Chronic lymphocytic leukemia
d.Acute myelomonocytic leukemia

Answer 8

a.Erythroleukemia

Question 9

9.Which enzyme stain is a marker for primitive lymphoid populations?
a.Nonspecific esterase
b.Sudan black B
c.Chloroacetate esterase
d. Terminal deoxynucleotidyl transferase

Answer 9

d. Terminal deoxynucleotidyl transferase

Question 10

10.Which staining procedure utilizes both a-naphthyl butyrate and naphthol-AS-D chloroacetate?
a. Sudan black B
b. Acid phosphatase
c.Periodic acid-Schiff
d. Combined esterase

Answer 10

d. Combined esterase

Question 11

11.Myeloperoxidase is sensitive to all of the following except:
a.Heat
b.Storage
c.Heparin
d.Methanol

Answer 11

c. Heparin

Question 12

12. What two stains parallel each other in separating acute myeloid leukemia from acute lymphoblastic leukemia?
    a. Acid phosphatase and alkaline phosphatase
    b. Nonspecific esterase and specific esterase
    c. Periodic acid-Schiff and Prussian blue
    d. Myeloperoxidase and Sudan black B

Answer 12

d. Myeloperoxidase and Sudan black B

Question 13

13. A medical technologist sees blasts on a peripheral blor e e e o da se and sudan laceB stains are per.nonspecific esterase stain using a-naphthyl butyrate This would indicate that the type of leukemia is:
    a. Acute lymphocytic leukemia
    b. Acute erythroleukemia
    c.Acute monocytic leukemia
    d. Chronic lymphocytic leukemia

Answer 13

c.Acute monocytic leukemia

Question 14

14. Which cytochemical stain is used to stain glycogen granules ? (positive is a bright red color).

a. Nonspecific esterase
b. Acid phosphatase
c. Myeloperoxidase
d.Periodic acid-Schiff

Answer 14

d.Periodic acid-Schiff