34. Applications of Flow Cytometry to Hematology and Hemostasis

Question 1

1. Which of the four main tasks related to flow cytometric analysis often requires the expertise of a specialty physician?

a. Sample preparation
b. Cytometer operation
c.Data analysis
d. Results interpretation

Answer 1

d. Results interpretation

Question 2

2.Antibodies binding the same antigen are classified in the same:
a.Isotype
b.ABO group
c.CD group
d.HLA group

Answer 2

c.CD group

Question 3

3.Which characteristics apply to monoclonal antibodies?
a. Purified from antisera
b.Derived from cloned, immortalized B-cell cultures
c.Highly specific and well characterized
d.High background staining

Answer 3

b.Derived from cloned, immortalized B-cell cultures
c.Highly specific and well characterized

Question 4

4. Which is not a major system in a flow cytometer?
   a.Threshold
   b.Fluidics
   c.Optics
   d.Electronics

Answer 4

a.Threshold

Question 5

5. An FSC/SSC plot displays two population, A and B population A is to the left of population B. Based on this information, which statement is true ?

a. Cell of population B are more granular than those in A
b. Cells of population A are larger than those in B
c. Cells of population A are smaller than those in B.
d. Cells in population A are more granular than those in B.

Answer 5

b. Cells of population A are larger than those in B

Question 6

6. This is used to restrict the data analysis to one population.

a. Compensation
b. Linear amplification
c. Gating
d. Logarithmic amplification

Answer 6

c. Gating

Question 7

7. Reviewing figures 34-7C and 34-8, what is the percentage of CD3-positive/CD4- negative lymphocytes ?

a. 9.07
b. 37.92
c 26.03
d. 13.21

Answer 7

c 26.03

Question 8

8. Reviewing Figures 34-7C and 34-8, which two quadrant statistics must be summed to obtain the percentage of CD3 positive lymphocytes ?

a. UL and UR
b. UR and LR
c. UL and LL
d. LL and LR

Answer 8

b. UR and LR

Question 9

9. Reviewing Figure 34-9, which region statistic would be used to measure the percentage of lymphocytes that is CD3-negative/CD4-positive ?

a. R3, % Gated
b. R3, % Total
c. R4, % Gated
d. R4, % Total

Answer 9

a. R3, % Gated

Question 10

10. Reviewing Figure 34-10, what is the percentage of CD3-negative lymphocytes ?

a. 5.09
b. 13.84
c. 39.72
d. 59.87

Answer 10

c. 39.72

Question 11

11. In comparing cell-cycle phases within a cell population, which of the following expressions are true with respect to DNA content ?

a. S phase <G2 + M phase
b. G2 + M phase > S phase
c. G2 + M phase > G0/G1 phase
d. S phase < G0/G1 phase

Answer 11

b. G2 + M phase > S phase
c. G2 + M phase > G0/G1 phase

Question 12

12. A sample contains 50 ul of blood and 40,000 reference beads. A 10, 000 event data file is collected and analyzed using region statistics. The following information was obtained. There were 1500 events for cell population X, and 1000 bead events in the data file. Calculate the absolute count per microliter of blood for population X.

a. 5.33
b. 1200
c. 8000
d. 120

Answer 12

a. 5.33
b. 1200

Question 13

13.What is monitored in HIV-positive individuals to deter-mine if antiviral treatment should begin?
a.CD3 percentage
b.CD8 absolute counts
c.CD8 percentage
d. CD4 absolute counts

Answer 13

d. CD4 absolute counts

Question 14

14.Which disease is not characterized by a reduction of T lymphocytes?
a.Severe combined immunodeficiency
b.X-linked hypogammaglobulinemia
c.Thymic hypoplasia
d.Acquired immunodeficiency disease syndrome

Answer 14

b.X-linked hypogammaglobulinemia

Question 15

15.Which statements are true regarding DNA content analysis?

a. It is used as adiagnostic test.
b. It has prognostic value for a limited number of hema-tologic malignancies.
c.It has prognostic value for a wide range of hemato-logic malignancies.
d.None of the above.

Answer 15

b. It has prognostic value for a limited number of hema-tologic malignancies.

Question 16

16.Which antibodies are used for hematopoietic progenitor cell enumeration?

a. CD34
b. CD25
c. CD10
d. CD45

Answer 16

a. CD34
d. CD45

Question 17

Anti-HgbF is used to detect:

a.Reticulocytes
b.Fetal erythrocytes
c.Platelets
d.Leukemias

Answer 17

b.Fetal erythrocytes

Question 18

18.Residual white blood cells are detected using:
a.Anti-HgbF
b.Propidium iodide
c.Phycoerythrin
d.Thiazole orange

Answer 18

b.Propidium iodide

Question 19

19.Paroxysmal nocturnal hemoglobinuria is detected by:

a. A decrease in CD59 staining intensity on erythrocytes
b. An increase in CD59 staining intensity on neutrophils
c.A decrease in CD59 staining intensity on neutrophils
d.A decrease in CD45 staining intensity on erythrocytes

Answer 19

a. A decrease in CD59 staining intensity on erythrocytes
c.A decrease in CD59 staining intensity on neutrophils

Question 20

20.Flow crossmatching detects:
a. Alloantibodies in the donor’s serum
b.Alloantibodies in the recipient’s serum
c.Cytotoxic T cells in the donor’s blood
d.a and b

Answer 20

b.Alloantibodies in the recipient’s serum

Question 21

21. Regarding bead-based assays, which of the following is NOT true ?

a. They require the use of special dyes that cannot be run on a clinical flow cytometer.

b. They rely on fluorescent beads with surface-bound antibody or antigen

c. They make use of reporter antibodies that bind specifically to the analyte.

d. They are capable of detecting multiple analytes simultaneously from one sample.

Answer 21

a. They require the use of special dyes that cannot be run on a clinical flow cytometer.