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*** Hematology & Fundamentals of Hemostasis 5th > 31. Hematology Methods > Flashcards

31. Hematology Methods Flashcards

1
Q
  1. Twenty microliters of blood are drawn into a unopette system for a WBC count. Fifty cells are counted on one side and 52 on the other side of the hemacytometer (4 large squares). Calculate the WBC count.

a. 12.5×10^9/L
b. 12.7×10^9/L
C. 12.0×10^9/L
d. 3.0×10^9/L

A

b. 12.7×10^9/L

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2
Q
  1. If a petient has a reticulocyte count of 8% with a hematocrit of 18%, what is the corrected reticulocyte count?
    a.20%
    b.2.3%
    c.3.2%
    d.8%
A

c.3.2%

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3
Q
  1. Which of the following errors will cause falsely elevated results for a centrifugal microhematocrit?

a.Reading of buffy coat with red cells
b. Incomplete sealing of capillary tubes
c.Allowing the centrifuge to stop without breaking
d.All of the above

A

d.All of the above

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4
Q

4.Which of the following factors affect the ESR?
a.Increased fibrinogen
b. Extreme poikilocytosis
c.Use of heparin as an anticoagulant
d.All of the above

A

d.All of the above

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5
Q

5.What type of hemoglobin electrophoresis would be best to separate hemoglobins S and D?

a. Cellulose acetate at alkaline pH
b.Citrate agar at acid pH
c. Either cellulose acetate or citrate agar may be used

A

b.Citrate agar at acid pH

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6
Q

6.What is the principle for the acid elution test for HbF?
a. HbF is resistant to acid elution; it can be precipitated and stained.
b.HbF is susceptible to acid elution; it can be dissolved and measured photometrically.
c. HbF is resistant to acid elution; it can be separated by aspirating the acid from the remaining hemoglobin.
d.HbF is susceptible to acid elution; it can be destroyed and the denatured hemoglobin can be detected by a color reaction.

A

a. HbF is resistant to acid elution; it can be precipitated and stained.

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7
Q

7.Which condition shoms increated osmotic fragility?

a. Hereditary spherocytosis
b. Sickle cell anernia
c.Acqivited hemolytic anetria
d. Hemolytic disease of the newborn

A

a. Hereditary spherocytosis

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8
Q

8.Whet is the basic principle of both the Ham test and the sugar watet test for PNH?

a. Complement-mediated RBC Iysis
b. Precipitation of abnormal RBCs
c. Vital staining of affected RBCs
d. Differential agglutination of RBCs

A

a. Complement-mediated RBC Iysis

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9
Q

9.Calculate the corrected WBC count for a smear contain-ing 20 NRBCs (WBC count=4500/uL)

a 4500/μL
b.375/μL
c.4480uL
d.3750/μL

A

d.3750/μL

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10
Q
  1. What is the principle of the cyanmethemoglobin method in the colorimhetric determination of hemoglobin?

a. Potassiuim fericyanide oxidizes hemoglobin to methe-moglobin,potassium cyanide converts methemoglobin to cyanmethemoglobin.

b. Potauium cyanide oxidizes hemoglobin to methemo-globin: potasstum fenricyanide converts methemoglo-

c.Potasiium fericyanide oxidixes hemoglobin to cyan-methemoglobin:cyanmethemoglobin degenerates to furm potassium Cyanide.

d.Polassium cyanide reduces hemoglobin to cyanmethe-moglobin.

A

a. Potassiuim fericyanide oxidizes hemoglobin to methe-moglobin,potassium cyanide converts methemoglobin to cyanmethemoglobin.

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11
Q

Electrophoresis can be defined as:

a. Movement of large particles through a column
b. Movement of small particles through a column
c. Movement of charged particles in an electric field
d. Tagging of a small molecule with a fluorescent protein

A

c. Movement of charged particles in an electric field

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12
Q
  1. Hematocrit can be defined as :

a. Volume occupied by white blood cells in a given volume of blood expressed as a percentage

b. Volume occupied by platelets in a given volume of blood expressed as a percentage

c. Volume occupied by red blood cells in a given volme of blood expressed as a percentage

d. Volume occupied by a stromal cells in a given volume of blood ex^ressed as a percentage

A

c. Volume occupied by red blood cells in a given volme of blood expressed as a percentage

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13
Q
  1. A patient presents with an MCV of 78 fL and an MCHC of 33%. How would this anemia be classified ?

a. Microcytic, normochromic

b. Normocytic, normochromic

c.Microcytic,hypochromic

d.Normochromic,hyperchromic

A

a. Microcytic, normochromic

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14
Q

14.What effect do sickle cells have on the ESR?
a.Increased
b.Decreased
c.Normal

A

b.Decreased

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15
Q

15.What is the principle of Isoelectric focusing?

a. PBS at a pH of 6 to 8 allow separation of abnormal hemoglobins based upon their pI
b. Ampholyte buffers at a pH of 6 to 8 allow separation of abnormal hemoglobins based upon their pl
c. PBS at a pl of 6 to 8 allow separation of abnormal hemoglobins based upon their pH
mpholyte buffers at a pI of 6 to 8 allow separation of abnormal hemoglobins based upon their pH

A

b. Ampholyte buffers at a pH of 6 to 8 allow separation of abnormal hemoglobins based upon their pl

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*** Hematology & Fundamentals of Hemostasis 5th (36 decks)

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