32. Principles of Automated Differential Analysis

Question 1

1. What is the main use of the leukocyte histogram differential?

a.Diagnostic laboratory test for hematology diseases

b. Research tool for the routine hematology laboratory

c. Replacement for the conventional manual differential

d. Screening tool for hematologic diseases

Answer 1

d. Screening tool for hematologic diseases

Question 2

2.AnRBChistogramthat shows an RBC distribution thatis shifted tothe left is characteristic of which of the following?

a.Macrocytosis
b.Anisocytosis
c.Microcytosis
d.Spherocytosis

Answer 2

c.Microcytosis

Question 3

3. The RDW reprsents a parameter that quantifies relative:
   a.Macrocytosis
   b.Anisocytosis
   c.Platelet size
   d.Microcytosis

Answer 3

b.Anisocytosis

Question 4

4.The MPV and PDW are parameters describing:

a.White blood cell size
b.Red blood cell size
c.Platelet size
d.Multiple cell size

Answer 4

c.Platelet size

Question 5

5.Which of the following best describes the principle of the white cell differential of Coulter LH Series instruments?

a.Laser and light scatter
b.Volume,cell size,and cytochemistry
c.Volume,conductivity,and light scatter
d. Laser and immunofluorescence

Answer 5

c.Volume,conductivity,and light scatter

Question 6

6.Which of the following best describes the principle of the Siemens ADVIA 120/2120 technology?

a. Electrical impedance, volume, and conductivity
b. Flow cytometry,cytochemistry,and light scatter
c.Chemiluminescence and laser cell counting
d.Cell volume and light scatter

Answer 6

b. Flow cytometry,cytochemistry,and light scatter

Question 7

7.In the WBC differential report generated by the Siemens ADVIA 120/2120 instruments,increased numbers of LUCs can include:

1)Variant lymphocytes
2)Promyelocytes
3)Blasts
4)Rubricytes

a.1,2,3
b.1,3
c.2,4
d.4 only

Answer 7

b.1,3

Question 8

8.Where does platelet enumeration take place in the Bayer ADVIA 120 hematology analyzer?

a.Peroxidase reaction chamber
b.RBC reaction chamber
c. Hemoglobin reaction chamber
d.Basophil reaction chamber

Answer 8

b.RBC reaction chamber

Question 9

9.Which of the following best describes the principle of Sysmex X-Series analyzers?

a.Impedance and light scatter
b.Cytochemistry and flow cytometry
c.Focused flow impedance and fluorescent flow cytometry
d.Conductivity and volume

Answer 9

c.Focused flow impedance and fluorescent flow cytometry

Question 10

10.Which of the following best describes the principles of CELL-DYN MAPSS Plus Fluorescent Analysis?

a.Focused flow impedance with multicolor fluorescence and multi-angle light scatter
b. Focused flow impedance and cytochemistry
c.Focused fow impedance and multi-angle light scatter
d. Electrical impedance and laser light scatter

Answer 10

a.Focused flow impedance with multicolor fluorescence and multi-angle light scatter

Question 11

11.What should be the basis for thedecisiontoaccept an automated leukocyte histogramdifferentialor to perfoe a conventional manual differential?

a. Each individual laboratorys established criteria for manual review of automated differential analysis
b.Availability of qualified technologists
c.Laboratory workload
d.Manufacturer’s recommendation

Answer 11

a. Each individual laboratorys established criteria for manual review of automated differential analysis