2: Introduction Flashcards
When do you see high troponin I?
Heart attack
When do you see high pro-BNP?
If large atria
Which cations does EDTA bind to?
Ca, and other divalent ions less strongly eg Mg
How does citrate work?
Chelates Ca
What do you get if you centrifuge a citrate tube?
Citrated plasma
How do you reverse the effect of citrate?
Add Ca
What do you use heparin to obtain?
Plasma
How does heparin work?
Through antithrombin and weakly binds to calcium
Which heparin salt do you use for plasma biochem?
Lithium
What sample do you usually use for biochem?
Serum in a gel separator
For which tests do you usually use serum?
Chemistry, bile acids, serology, hormones
Which tube should you use if you can’t separate the glucose?
Oxalate
What happens if you leave non-separated samples out?
K+ leaks from cells, glucose will be used up
Which samples must be frozen?
e.g. PTH, ACTH, insulin
Which values can become high if transported at the wrong temperature?
pro-BNP, TSH, IGF-1
Which values can become low if transported at the wrong temperature?
PTH, aldosterone, vasopressin, T3, cortisol
What is the stress response?
Increased neutrophils, decreased lymphocytes
Which values can GA affect?
CBC, chem, RBC/WBC count, induces liver enzymes
How long does dexamethason last?
36 hours
How does does pred last?
12 hours
Why does giving steroids look like pancreatitis?
Decreases amylase, increases lipase
What other values can steroids affect?
Glucose and ALP
What value an bromide therapy interfere with?
Calcium - gives false high
Which values can phenobarb affect?
Induces ALP and increases liver enzymes because causes bile duct epithelium hyperplasia
What values can diuretics affect?
Increases CBC, hyperkalaemia, increases protein/albumin, looks like pre-renal azotaemia
Which parameters can haemolysis affect?
K+, LDH, ALP, P, interferes with colorimetric assays
