1: Introduction Flashcards

1
Q

Which tube for haematology?

A

EDTA

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2
Q

Which tube for biochem?

A

Serum or heparin (which gives plasma when separated)

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3
Q

Which tube for coagulation?

A

Citrate

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4
Q

Which tube for glucose?

A

Fluoride

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5
Q

Which tubes to get plasma?

A

Heparin or citrate

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6
Q

What does plasma contain?

A

Fibrinogen but no cells (because has not clotted)

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7
Q

What does serum contain?

A

No cells or fibrinogen (because has clotted)

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8
Q

How do you get serum?

A

Gel separator

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9
Q

What are the three causes of lipaemia?

A

Insufficient fasting, endocrine disease, inherited hyperlipidaemia

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10
Q

How often do you do internal quality control?

A

Daily using control samples

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11
Q

How often do you do external quality control?

A

Monthly

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12
Q

What happens to platelet count in old samples?

A

Falls as stick to each other

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13
Q

What happens to MCV in old samples?

A

Increases as RBCs swell

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14
Q

What happens to biochemistry in old samples?

A

RBCs use up glucose, K+ escapes from them, Ca2+ may fall

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15
Q

Which breed has high PCV +/- low neutrophils/platelets?

A

Greyhounds

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16
Q

Which breed has low platelets?

17
Q

Which breed has high eosinophils due to gut flora?

18
Q

Which three parameters can bone growth due to age affect?

A

Ca, PO4, ALP

19
Q

Which parameters are different in young animals?

A

Low alb/glob, low PCV, high lymphocytes

20
Q

Which changes can steroids cause?

A

Elevated ALP and lipases, mild hyperglycaemia, high neutrophils and monocytes, low lymphocytes and eosinophils

21
Q

Which parameters can sedatives affect?

A

Lower PCV and white cell count

22
Q

Which parameters can IV fluids affect?

A

Lower PCV and proteins

23
Q

Which parameters can stress/adrenaline affect?

A

High neutrophils, low lymphocytes, high PCV, high glucose in cats

24
Q

What does height of pulse show on an analyser?

25
What does frequency of pulse show on an analyser?
Platelet and RBC count
26
Which type of cytometer can distinguish cell granularity/complexity?
Flow - can distinguish RBC vs platelet and give better WBC counts
27
What is the best way of getting a WBC differential count?
Film exam
28
What is the best type of cytometer for a platelet count?
Flow (better than impedence)
29
For what % of animals do reference ranges apply?
95%
30
What may a nucleated RBC be miscounted as?
Leucocyte
31
How does heparin exert its action?
Through antithrombin