1: Introduction Flashcards
Which tube for haematology?
EDTA
Which tube for biochem?
Serum or heparin (which gives plasma when separated)
Which tube for coagulation?
Citrate
Which tube for glucose?
Fluoride
Which tubes to get plasma?
Heparin or citrate
What does plasma contain?
Fibrinogen but no cells (because has not clotted)
What does serum contain?
No cells or fibrinogen (because has clotted)
How do you get serum?
Gel separator
What are the three causes of lipaemia?
Insufficient fasting, endocrine disease, inherited hyperlipidaemia
How often do you do internal quality control?
Daily using control samples
How often do you do external quality control?
Monthly
What happens to platelet count in old samples?
Falls as stick to each other
What happens to MCV in old samples?
Increases as RBCs swell
What happens to biochemistry in old samples?
RBCs use up glucose, K+ escapes from them, Ca2+ may fall
Which breed has high PCV +/- low neutrophils/platelets?
Greyhounds
Which breed has low platelets?
CKC
Which breed has high eosinophils due to gut flora?
GSDs
Which three parameters can bone growth due to age affect?
Ca, PO4, ALP
Which parameters are different in young animals?
Low alb/glob, low PCV, high lymphocytes
Which changes can steroids cause?
Elevated ALP and lipases, mild hyperglycaemia, high neutrophils and monocytes, low lymphocytes and eosinophils
Which parameters can sedatives affect?
Lower PCV and white cell count
Which parameters can IV fluids affect?
Lower PCV and proteins
Which parameters can stress/adrenaline affect?
High neutrophils, low lymphocytes, high PCV, high glucose in cats
What does height of pulse show on an analyser?
Cell size
What does frequency of pulse show on an analyser?
Platelet and RBC count
Which type of cytometer can distinguish cell granularity/complexity?
Flow - can distinguish RBC vs platelet and give better WBC counts
What is the best way of getting a WBC differential count?
Film exam
What is the best type of cytometer for a platelet count?
Flow (better than impedence)
For what % of animals do reference ranges apply?
95%
What may a nucleated RBC be miscounted as?
Leucocyte
How does heparin exert its action?
Through antithrombin
