Transcription and RNA Processing

Question 1

Central Dogma

Answer 1

DNA- (transcription)- RNA -(translation) - proteins

Question 2

Eukaryotic Polymerases

Answer 2

I: rRNA
II: mRNA
III: tRNA

Question 3

Prokaryotic Polymerase

Answer 3

RNA I: makes all 3 kinds of RNA

Question 4

Eukaryotic Promoters

Answer 4

Enhancer, CAAT box, GC box, TATA box (-25), Initiator (+1), Downstream promotor element (+25)

Question 5

Prokaryotic Initiation and elongation

Answer 5

RNA polymerase, bound to sigma factor, initiates, elongates, and then terminates by rho terminators

Question 6

Eukaryotic Initiation

Answer 6

TFIID binds TATA box
recruits TF, RNA POL II, mediator
form basal transcrtiption apparatus

Question 7

pre mRNA processing

Answer 7

5’ capping
3’ polyA tailing
RNA splicing (require consensus sequences)

Problems with splice site leads to cancers, dementia, epilepsy, Marfan, Gaucher, thalassemias (hereditary anemias, problem with Hb bc of splicing)

Question 8

post translational processing

Answer 8

methylation, etc.