Enzyme Kinetics Flashcards
1
Q
Affinity
A
strength of interaction between E and S
2
Q
Specificity
A
ability of E to recognize S
3
Q
Kcat
A
efficiency: concentration of S molecules one enzyme can convert per second
4
Q
Km
A
measure of affinity
[S] and 1/2Vmax
small: high affinity
5
Q
Michaelis Menten vs Lineweaver Burke
A
MM: good for calculatng Km and Vmax
LB: good for two substrate data or inhibition
6
Q
Lineweaver Burke
A
reciprocol: slope = Km/Vmax
x int: -1/Km
y int: 1/vmax
7
Q
Reversible inhibitors
A
bind to free E prevent S binding
bind ES complex
slow down reaction
8
Q
Irreversible inhibitors
A
permanently shut off enzyme (toxins)
9
Q
Competitive inhibition
A
MM: no change Vmax, increase Km
LB: intersect at y axis
10
Q
Noncompetitive Inhibition
A
MM: decreasae Vmax, no change Km
LB: lines intersect on x axis
