Molecular Techniques Flashcards
1
Q
Southern Blot
A
DNA
Detect large changes in DNA
Point mutations associated with restriction sites
2
Q
Northern blot
A
RNA
Measures mRNA amount and size
3
Q
Western blot
A
Proteins
4
Q
ASO (probes)
A
DNA
Small changes in DNA
Used with southern blots
5
Q
Microarray
A
CDNA or genomic DNA, proteins
Measures expression
6
Q
ELISA
A
Proteins
Detects antigens or antibodies
Low sensitivity (false positives) prefer ELISA
7
Q
PCR amplification
A
DNA replication
SDS page gell: smaller pieces go farther
8
Q
RFLP: restriction fragment length polymorphism
A
Detects SNPs and DNA length
