Test 2: filoviruses

Question 1

Rhabdoviruses are —

Answer 1

enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies)
makes 5 proteins

Question 2

what shape are rhabdoviruses

Answer 2

bullet-shapes

Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies)

Question 3

N —— (encapsidates the RNA)
P — (component of polymerase)
M —- (assembly and budding)
G —- (attachment)
L — (RNA-dependent RNA polymerase)

Answer 3

Nucleocapsid protein
Phosphoprotein
Matrix protein
Glycoprotein
Large protein

5 proteins make by rhadboviruses (rabies,VSV)

Question 4

where does rhadboviruses replicate

Answer 4

in the cytoplasma
(negri bodies)

Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies)

Question 5

two genus of rhabdoviruses

Answer 5

lyssavirus - rabies
vesiculovirus - vesicular stomatitis - foot and mouth disease look alike

Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies, vesicular stomatitis)

Question 6

how to vaccinate wild life for rabies

Answer 6

Recombinant Vaccinia Virus expressing the Rabies Virus G Glycoprotein.

Raboral or V-RG

Question 7

wild life rabies vaccine is a —

Answer 7

(live) recombinant vaccina virus that has the Rabies G glycoprotein

Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies, vesicular stomatitis)

Question 8

pathogenesis of rabies

Answer 8

transferred in saliva through bite wound
slow travel to nerves
when in brain, multiplies rapidly and moves to salivary gland →show clinical signs
death in 7 days of symptoms

Question 9

negri bodies are found in cytology of what virus

Answer 9

rabies

Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies, vesicular stomatitis)

Question 10

how long is the incubation period of rabies

Answer 10

variable
depends how long to get to the brain

Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies, vesicular stomatitis)

Question 11

what test to detect rabies virus antigen

Answer 11

brain specimen
looking for fluorescent N(nucleocapsid) protein

Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies, vesicular stomatitis)

Question 12

In the Salivary Glands, Rabies virus is formed by —

Answer 12

budding on the apical plasma membranes.
Cell free virus

Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies, vesicular stomatitis)

Question 13

In the Brain, Rabies virus is formed by —

Answer 13

crawl from cell to cell
budding on internal cellular membranes and the virus is trapped

Question 14

3 stages of classical rabies

Answer 14

Prodromal Phase - lasts 2-10 days, fever, headache, malaise, fatigue and anorexia.
Sensory Excitation Phase - lasts 2-7 days, neurological symptoms develop, agitation, thrashing, convulsions, and muscle spasms.
Coma + Paralysis Phase - Mental status deteriorates, sudden cardiac or respiratory arrest, coma.

also called furious rabies - 80%

rhabdo virus

Question 15

dumb or paralytic rabies

Answer 15

Patient may experience prodromal symptoms (fatigue, headache)
and then enter a paralysis phase(cardiac and respiratory arrest, coma).
skips the frenzy phase

Question 16

how to treat rabies in a human

Answer 16

first aid- clean wound
Human Diploid Cell Vaccine- killed vaccine
Human Rabies Immune Globulin (HRIG)- given at site different from vaccine

Question 17

vesicular stomatitis virus cause —

Answer 17

foot and mouth disease look alike
salivation,lameness, lesions in mouth and on feet

Mononegavirales Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies, vesicular stomatitis)

Question 18

how is vesicular stomatitis virus spread?

Answer 18

breaks in the skin
maybe by sandflies

Mononegavirales Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies, vesicular stomatitis)

Question 19

vesicular stomatitis virus causes — in humans

Answer 19

mild flu like symptoms

Mononegavirales Rhabdo- enveloped, bullet shaped, with a single external peplomer(G protein and a helical nucleocapsid consisting of a linear negative sense ssRNA (rabies, vesicular stomatitis)

Question 20

Filoviridae are —

Answer 20

subclass of mononegavirales (rabdo also in this family)

non-segmented negative sense ssRNA (ebola)

Question 21

vaccine for elboa

Answer 21

VSV vector vaccine with piece of ebola added on

Filoviridae- non-segmented negative sense ssRNA (ebola)

Question 22

— is a catagory A, BSL4 pathogen

Answer 22

ebola

filoviridae- non-segmented negative sense ssRNA (ebola)

Question 23

reservoir for ebola

Answer 23

fruit bats

filoviridae- non-segmented negative sense ssRNA (ebola)

Question 24

how do humans get ebola

Answer 24

fruit bat to other animal that humans eat or have close contact
human-human- virus spread through contact to bodily fluids

filoviridae- non-segmented negative sense ssRNA (ebola)