Respiratory Burst

Question 1

What is it?

Answer 1

an intracellular way of killing microbes/pathogens

Question 2

How does it work?

Answer 2

1) O2 consumption increases
2) Hexose monophosphate shunt (HMPS) activity is stimulated
3) H2O2 production increases
(denatures essential enzymes and transport proteins)
4) superoxide anion is produced

Question 3

So what principle effect does it have, and then what other antimicrobial effects does it produce?

Answer 3

production of superoxide anion

also produces H2O2 and facilitates hexose mannophosphate shunt

Question 4

SOA: three steps

Answer 4

SOA –> H202 via superoxide dismutase –> H202 is broken down by CATALASE –> free radicals

Question 5

what enzyme is responsible for breaking down H202?

Answer 5

catalase

Question 6

What is produced via H202 breakdown?

Answer 6

hydroxide radical and a singlet oxygen

Question 7

Myeloperoxidase

Answer 7

catalyzes H2O2 via toxic peroxidation on the surface of microorganisms

produces hypochlorite

Question 8

Hypochlorite

Answer 8

by product of toxic peroxidation via myeloperoxidase enzyme

Question 9

what makes hypochlorite significant

Answer 9

it is more antimicrobial alone than each of the three components (halide, H202, myeloperoxidase

Question 10

O2 –> X –> X –> X –> X –> X + X

Answer 10

O2 –> hexose monophosphate shunt begins to –> H202 –> breaks down into superoxide anion –> superoxide dismutase converts it back into H2O2 –> catalase breaks it down into singlet oxygen and hydroxyl radicals