Proteins and Permeability Flashcards
what are the six major functions of membrane protein?
1) transport
2) Enzymatic activity
3) Cell to cell recognition
4) Attachment to cytoskeleton & Extra-cellular matrix
5) Single transduction
6) Intracellular joining
What moelcules have some permeability?
Some permeability to water molecules and small uncharged molecules like carbon dioxide and oxygen
What molecules aren’t permeable?
ions, K+,CA 2+,NA +, Cl-, HCO3-, small hydrophilic molecules, macromolecules (protein/RNA) and sugar (fructose and glucose)
What is diffusion?
Due to random motion over time, molecules become more equally distributed, no energy is required. It eliminates a con. gradient so molecules can cross
What is osmosis?
Diffusion of water through a selectively permeable membrane from an area of low con. into an aqueous compartment containing absolute at a higher concentration (across)
When does osmosis occur?
It occurs when a solute cannot cross the cell membrane, so the water moves instead
Passive transportation
moves from an area with a high con, to an area with low con.
What is facilitated diffusion?
Passive transport aided by proteins, down the con. gradient via transport protein
What is active transport?
Sodium and potassium pump moves ions against their con. gradient ( requires energy)
Lower con –> to a higher con.
USES ATP
Co transport?
sodium and glucose ions move into the cell through a co-transporter protein glucose with and sodium against
Osmotica and osmolarity
Glucose stays the same, ions break up, if you have two times then add together
Questions to ask doing osmolarity Qs
1) can the solute cross the membrane w/passive diffusion (it doesn’t count)
2) osmosis
3) When a solute dissolves, does a molecule stay together or split into ions?
Osmolarity =?
(osm/L)= molar con X no. moles or ions in solution
What is the tonicity?
The ability of a solution to cause a cell to gain/loose water
Define isotonic?
Solution concentration is the same as the inside of the cell, no net water movement across the plasma membrane.