Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Parasitology > ParaLab - Diagnostic Para > Flashcards

ParaLab - Diagnostic Para Flashcards

1
Q

simplest and easiest technique to facilitate detection of intestinal parasites that infected subjects pass in their feces

A

direct fecal smear

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

used to directly detect presence of protozoans and helminths?

A

DFS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what solution in the DFS is used for the detection of trophozoite and larva motility?

A

NSS

Normal Saline Solution

  • may also detect RBCs, Leukocytes, Charcot-Leydens
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What solution do u use in DFS to demonstrate parasite structure?

A

Lugol’s Iodine

Iodine destroys trophozoites and organisms present

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What solution do you use when u want to detect protozoan cysts?

A

Lugol’s

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How many drops each should you use in NSS & Lugol’s?

A

1 drop each

NSS muna b4 Lugol’s coz iodine is toxic sa parasites

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

this is the qualitative analysis ng laboratory diagnosis since parasite identification is employed. what test is it?

A

DFS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

This is the quantitative part ng Lab Diagnosis tests. It is used to count eggs of STHs.

A

Kato-Katz Technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what is egg-counting used for?

A
  • assess efficacy of treatment
  • correlate severity of disease w/ intensity of infection
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

this is the manner of reporting when using Kato-Katz technique

A

epg

eggs per gram stool

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what are the STHs?

A
  • H = ookworm
  • A = scaris lumbricoides
  • T = richuris trichiura
  • S = trongyloides

Schistosoma is not an STH pero nabibilang siya sa katokatz; Strongy no to kato afaik

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

for how many hours should you soak cellophane in the clearing agent first?

A

for 24 hours

if cellophane isn’t color green, add malachite green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

where do you soak the green-colored cellophane?

what’s the clearing agent used?

A

glycerol-glycerine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

why do we use green cellophane or malachite green?

A

other colors are strenuous. para masarap sa mata

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

steps in Kato-Katz

no need to memo this. just understand

A
  1. sieve stool using nylon screen
  2. scrape stool
  3. prep slide and template w/ hole then pahid stool then meron na sa slide
  4. place now the soaked cellophane then flatten by flipping or wine cork
  5. set aside for 1 hour so glycerol (clearing agent) may do its part
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

In Kato-Katz, hookworm eggs clear rapidly and won’t be visible after ____ to ____ mins

A

30 to 60 mins

so u need to leave slide w/ cellophane for that long lang

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Schistosome eggs in Kato-Katz procedure is preferable examined within how many hours?

A

24 hours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

True or False

Ascaris lumbricoides and Trichuris trichiura eggs will still be visible after many months in DFS

A

False, in Kato-Katz

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

for a 9 x 1 mm of template, how many grams of stool will u get and how much will u multiply that to get the epg?

A

50mg; x20

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

for a 6 x 1.5 mm of template, how many grams of stool will u get and how much will u multiply that to get the epg?

A

41.7 mg; x24

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

for a 6.5 x 0.5 mm of template, how many grams of stool will u get and how much will u multiply that to get the epg?

A

20mg; x50

pansin niyo, mg is indirectly proportional to amt multiplied bababhawdbaa xori i miss p6

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Hookworm classification of intensity of infection for Kato-Katz

A

light< 2k-4k >=high

lesser than 2k is light, moderate is gitna. greater than or equal to 4k is high

23
Q

Ascaris lumbricoides classification of intensity of Infection for Kato-Katz

A

light< 5k-50k >=heavy

lesser than 5k is light; between is moderate; greater than or equal to 50k is high

24
Q

Trichuris trichiura classification of intensity of Infection for Kato-Katz

A

light< 1k-10k >= heavy

lesser than 1k is light and gr8r than or = to 10k is heavy

25
Q

Schistosome spp. classification of intensity of Infection for Kato-Katz

A

light< 100-400 >=heavy

26
Q

what is recognized as the golden standard of malaria diagnosis?

A

Thick and Thin smear

27
Q

In thick and thin smears, what type of blood is preferred?

A

capillary blood

thru puncture

28
Q

after collecting drops of blood for thick smear, what do you do?

A

dehemoglobinize by dipping in water

29
Q

this type of smear is used for screening purposes.

A

thick smear

used because mas maraming amt of blood.

30
Q

why do we dehemoglobinize thick smears?

A

so that hemoglobin will not obscure parasites

ghost cell (ata) kasi hanap natin

31
Q

what will we do after we remove hemoglobin in thick smears?

A

stain with Giemsa (pH 7.2)

remember that basic stains adhere to acidic parts of d cell (nucleus kasi andon DNA/RNA deoxy…. acid)

32
Q

this smear is used for the determination of parasite species.

or “confirmatory” but madam diana doesn’t want confirmatory

A

thin smear

allows us to determine parasite present and is more specific

33
Q

after we smear the thin one, what do we do next?

A

fix with methanol

then stain w/ Giemsa after

34
Q

when do we collect thick and thin smears?

A

at the height of fever (during fever)

35
Q

average diameter of blood for thick smear

A

1-2 cm

for thin, feathered dapat and smear must cover 1/3 of the slide

36
Q

formula for %parasitemia

A

%parasitemia = (parasitized/total) x 100

37
Q

this laboratory diagnosis technique is used for the diagnosis of Enterobiasis and Taeniasis

A

Cellophane swab

or perianal swab

38
Q

Cellophane swab is only used for what parasite since its stool recovery is poor (<5%)

A

Enterobius vermicularis

39
Q

time of collection for cellophane swab

A

early in the morning b4 washing or defecation

40
Q

MOT of Enterobius vermicularis

A
  • fecal-oral
  • inhalation of eggs
41
Q

during what time of the day does Enterobius vermicularis lay its eggs in the perianal region?

A

during night

42
Q

Enterobiasis is characterized by severe itching in the perianal region and this is called?

A

pruritis ani

43
Q

this laboratory diagnosis technique is used to detect number of parasites present in stool and to remove debris that masks parasite

A

Concentration

44
Q

two types or methods of concentration

A
  1. Flotation
  2. Sedimentation
45
Q

In sedimentation, we should produce a solution with a ____ specific gravity than the stool sample so that parasite will sink

lower or higher

A

lower

mas mataas dpt spec grav ni stool so that it’ll sink

46
Q

In flotation, we should produce a solution with a ____ specific gravity than the stool sample so that parasite will float

lower or higher

A

higher

mas mababa spec grav ng stool will allow it to float

47
Q

what life cycle stage of the protozoan will be destroyed when u use FEACT?

A

trophozoites

48
Q

Conc techniques may use what type of stool specimen?

fresh or fixed?

A

fresh or fixed

49
Q

ethyl acetate in FEACT is for what?

A

lipid dissolving

50
Q

two types of solutions that may be used in flotation?

A
  1. NaCl sol’n
  2. ZnSO4 sol’n
51
Q

spec grav of sodium chloride solution

A

1.20

52
Q

spec grav of zinc sulfate solution

A

1.35

53
Q

in flotation, all will rise except what?

A

operculated eggs

  • operculated = trematode eggs & some cestodes
  • too fragile daw kasi masisira smth pagkakaalala q

Parasitology (25 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions