Amoeba and Flagellates questionnaire (from book)

Question 1

Rounded cysts were found in a fresh stool preparation. The mononucleated cysts measured about 5 to 20 µm in diameter and contained a large glycogen vacuole. What is the most likely identification of this cyst?
a. Endolimax nana
b. Entamoeba histolytica
c. Giardia lamblia
d. Iodamoeba bütschlii

Answer 1

D. Iodamoeba butschlii

Question 2

Which of the following correctly describe giardiasis?
a. Attachment to the epithelia is facilitated by protein secretions
b. Giardia is one of the most common opportunistic infection in immunosuppressed patients.
c. The infection is initiated by the ingestion of oocyst.
d. Trophozoites attached to the host is mediated by the ventral disc.

Answer 2

d. Trophozoites attached to the host is mediated by the ventral disc

Question 3

Which of the following is the specimen of choice to diagnose PAM?
a. Corneal scrapings
b. CSF
c. Sputum
d. Stool

Answer 3

b. CSF

Question 4

This virulence factor of Entamoeba histolytica is known to cause cellular protein degradation in the basement membrane of host intestinal mucosa. It is known as:
a. Amebapore
b. Gal/GalNAc-binding-lectin
c. Cysteine proteinase
d. Hyaluronidase

Answer 4

C. Cysteine proteinase

Question 5

Cyst formation of E. histolytica occurs only within which of the following:
a. Intestines
b. Stomach
c. Lungs
d. Liver

Answer 5

a. Intestines

Question 6

Upon examination of stool of a patient, a medical technologist observed the following:
i. Trophozoites motility is progressive and rapid
ii. With fine, evenly distributed granules in the peripheral chromatin; darkly stained
iii. Contains small and compact karyosome centrally locate in the nucleus
iv. ∼8 to 10 µm diameter trophozoites

These are attributed to which of the following protozoans?
a. Entamoeba histolytica
b. Entamoeba dispar
c. Entamoeba hartmanni
d. Entamoeba coli

Answer 6

c. Entamoeba hartmanni

Question 7

This commensal has a pear-shaped trophozoite with three anterior flagella, a nucleus and a prominent shepherd’s crook-like cytostome
a. Trichomonas vaginalis
b. Giardia lamblia
c. Chilomastix mesnili
d. Trichomonas hominis

Answer 7

c. Chilomastix mesnili

Question 8

Habitat: Trophozoites in the urogenital system
Infective stage: Trophozoite
Size: 5 to 18 um
Flagella: 4 anterior
Undulalating membrane: extends half the length of the body
Axostyle: present

The following descriptions are attributed to:
a. Dientamoeba fragilis
b. Trichmonas vaginalis
c. Chilomastix mesnili
d. Retortamonas intestinalis

Answer 8

b. Trichomonas vaginalis

Question 9

A student came from a 1-month visit in USA from April to May. She was then hospitalized for she complained of constant headaches, purulent nasal discharge. She was admitted to the hospital with a diagnosis of bacterial meningitis and died 5 days later.

Which of the following parasites should have been
considered in the diagnosis?
a. Balamuthia spp.
b. Acanthamoeba spp.
c. Entamoeba histolytica
d. Naegleria fowleri

Answer 9

d. Naegleria fowleri

Question 10

Fresh stool samples are requested specifically for the recovery of:
a. Cysts
b. Motile trophozoites
c. Cysts and trophozoites
d. Degenerated proglottid

Answer 10

b. Motile trophozoites

Question 11

These are known as the energizing structures of flagellates:
a. Flagellum
b. Cytostome
c. Median bodies
d. Axostyle

Answer 11

c. Median bodies

Question 12

A patient is suspected of having amoebic dysentery. Upon microscopic examination of a fresh fecal specimen for ova and parasites, the following data were obtained:
i. Trophozoites of about 25 µm in diameter
ii. With unidirectional and progressive motility
iii. Mononucleated with evenly distributed peripheral chromatin
iv. Finely granular cytoplasm

a. Entamoeba histolytica
b. Entamoeba coli
c. Iodamoeba bütschlii
d. Endolimax nana

Answer 12

a. Entamoeba histolytica

Question 13

A sexually active woman complains of vaginal itching and purulent vaginal discharge. To verify trichomoniasis, which of the following tests should be done?

a. Direct fecal smear examination
b. Enzyme Linked Immunosorbent Assay (ELISA)
c. Urine analysis
d. Wet mount examination of vaginal fluid

Answer 13

d. Wet mount examination of vaginal fluid

Question 14

Infection of this free-living amoeba typically occurs in healthy individuals causing CNS, eye, and disseminated infections:
a. Acanthamoeba
b. Balamuthia
c. Naegleria
d. Sappinia

Answer 14

a. Acanthamoeba

Question 15

Cysts of Entamoeba coli typically have:
a. A large glycogen vacuole
b. Centrally located karyosome
c. Ingested RBCs
d. Splinter shaped chromatoidal bars

Answer 15

d. Splinter shaped chromatoidal bars

Question 16

Eye infections with Acanthamoeba spp. have most commonly been traced to:
a. Use of other’s sunglasses
b. Failure to remove contact lenses while swimming
c. Use of contaminated lens care solutions
d. Application of eye make up

Answer 16

c. Use of contaminated contact lenses

Question 17

Motility of Entamoeba coli

Answer 17

Sluggish and nondirectional

Question 18

Motility of Entamoeba hartmanni

Answer 18

Progressive and rapid

Question 19

Motility of Giardia lamblia

Answer 19

Falling leaf motility

Question 20

Motility of Trichomonas vaginalis

Answer 20

Jerky and rapid