P7 IMMUNOLOGY LAB

Question 1

what is your diagnosis

Answer 1

Diffuse bilateral Interstitial Infiltrates secondary to Pneumocystis jiroveci infection

Question 2

stain used for detection of nuclei of trophozoites and intracystic stages in Pneumocystis jiroveci

Answer 2

Giemsa stain

Question 3

Identify the organism

Answer 3

Pneumocystis jiroveci

Question 3

Sample used for Pneumocystis jiroveci

Answer 3

Bronchopulmonary secretions

Question 4

Identify the organism and what stain is used

Answer 4

Pneumocystis jiroveci
Groccot’s methenamine silver stain

Question 5

what organism is causing this clinical presentation

Answer 5

herpes simplex & herpes zoster

Question 6

most common organism associated with PTLD

Answer 6

Epstein barr virus

Question 6

Identify the organism & what can you see

Answer 6

ballooning effect by HSV

Question 7

common clinical sign of the tongue seen with EBV

Answer 7

Hairy tongue

Question 8

Identify the disease & organism causing it

Answer 8

CMV retinitis

Question 9

Identify the organism

Answer 9

CMV inclusion bodies

Question 10

very sensitive gol standard test for CMV

Answer 10

cell culture

Question 11

a procedure in which An antibody labeled with fluorescein is conjugated with the monoclonal antibody against pp65

Answer 11

CMV Antigenemia Assay

Question 12

what test is shown

Answer 12

CMV antigenemia test

Question 13

a polyoma virus in the same family of viruses as human papilloma and JC virus.

Answer 13

BK virus

Question 14

transmission of BK virus

Answer 14

via respiratory secretions

Question 15

BK organism feature

Answer 15

Double-stranded DNA virus

Question 16

gold standard in BK virus

Answer 16

renal biopsy

Question 17

Differential diagnosis of BK virus

Answer 17

Other viral infections
Acute or chronic rejection
Malignancy

Question 18

Four clinical syndromes of atypical mycobacteria

Answer 18

• pulmonary disease
• lymphadenitis
• skin or soft tissue disease
• disseminated disease in AIDS.

Question 19

what stain is used

Answer 19

Ziehl-nielsen stain

Question 20

agar used for M.Tuberculosis

Answer 20

L-J agar

Question 21

an intestinal parasitic pathogen in patients with HIV infection

Answer 21

Cryptosporidium spp

Question 22

most important Clinical manifestation seen with Cryptosporidium spp

Answer 22

watery diarrhea

Question 23

Identify the organism

Answer 23

Cryptosporidium spp

Question 24

Identify the organism

Answer 24

aspergillus

Question 25

Describe what is shown

Answer 25

Aspergillus niger in a cavitary lung lesion showing both hyphae and conidial head

Question 26

agar used for fungi is

Answer 26

sabourauds agar

Question 27

Identify what is seen & what is the organism

Answer 27

-extensive granulomatous inflammation. Macrophages frequently contain “clusters of grapes” in the cytoplasm
- organism : histoplasma capsulatum

Question 28

Identify the organism

Answer 28

Cryptococcus neoformans (budding )

Question 29

stain used to detect Cryptococcus neoformans

Answer 29

india ink stain

Question 30

What was the common source of the toxoplasmosis in an transplanted patient

Answer 30

Reactivation of toxoplasmosis

Question 31

How do you diagnose toxoplasmosis?

Answer 31

Serology : Antibody for Toxoplasma : IgG, IgM, IgG avidity test