P7 IMMUNOLOGY LAB Flashcards
what is your diagnosis
Diffuse bilateral Interstitial Infiltrates secondary to Pneumocystis jiroveci infection
stain used for detection of nuclei of trophozoites and intracystic stages in Pneumocystis jiroveci
Giemsa stain
Identify the organism
Pneumocystis jiroveci
Sample used for Pneumocystis jiroveci
Bronchopulmonary secretions
Identify the organism and what stain is used
Pneumocystis jiroveci
Groccot’s methenamine silver stain
what organism is causing this clinical presentation
herpes simplex & herpes zoster
most common organism associated with PTLD
Epstein barr virus
Identify the organism & what can you see
ballooning effect by HSV
common clinical sign of the tongue seen with EBV
Hairy tongue
Identify the disease & organism causing it
CMV retinitis
Identify the organism
CMV inclusion bodies
very sensitive gol standard test for CMV
cell culture
a procedure in which An antibody labeled with fluorescein is conjugated with the monoclonal antibody against pp65
CMV Antigenemia Assay
what test is shown
CMV antigenemia test
a polyoma virus in the same family of viruses as human papilloma and JC virus.
BK virus
transmission of BK virus
via respiratory secretions
BK organism feature
Double-stranded DNA virus
gold standard in BK virus
renal biopsy
Differential diagnosis of BK virus
Other viral infections
Acute or chronic rejection
Malignancy
Four clinical syndromes of atypical mycobacteria
- pulmonary disease
- lymphadenitis
- skin or soft tissue disease
- disseminated disease in AIDS.
what stain is used
Ziehl-nielsen stain
agar used for M.Tuberculosis
L-J agar
an intestinal parasitic pathogen in patients with HIV infection
Cryptosporidium spp
most important Clinical manifestation seen with Cryptosporidium spp
watery diarrhea
Identify the organism
Cryptosporidium spp
Identify the organism
aspergillus
Describe what is shown
Aspergillus niger in a cavitary lung lesion showing both hyphae and conidial head
agar used for fungi is
sabourauds agar
Identify what is seen & what is the organism
-extensive granulomatous inflammation. Macrophages frequently contain “clusters of grapes” in the cytoplasm
- organism : histoplasma capsulatum
Identify the organism
Cryptococcus neoformans (budding )
stain used to detect Cryptococcus neoformans
india ink stain
What was the common source of the toxoplasmosis in an transplanted patient
Reactivation of toxoplasmosis
How do you diagnose toxoplasmosis?
Serology : Antibody for Toxoplasma : IgG, IgM, IgG avidity test
