P4 BIOCHEMISTRY Flashcards
replicating molecule used in recombinant DNA technology
bacterial plasmid
they restrict growth of certain
bacterial viruses called bacteriophages
Restriction enzymes
2 important tools used in preparing chimeric DNA molecules
Restriction enzymes and DNA-Ligase
2 types of vectors
Cloning vectors
Expression vectors
enzyme that can be used for direct blunt-end ligation
T4 DNA ligase
Examples of Cloning vectors
plasmids, phages or cosmids
a process that generates large number of identical DNA molecules
Cloning
Small, circular, duplex DNA molecules, naturally confer antibiotic resistance to host cell, used as cloning vectors
Bacterial Plasmids
Structure that Has linear DNA to which foreign DNA can be inserted at specific sites
Phages
Phage accept DNA inserts of
10 -20 Kb
a structure that combines features of both plasmid and phage
Cosmids
Cosmids can accept DNA inserts of
35-50 kp
Bacterial Artificial Chromosome can accept DNA inserts of
50 - 250 kb
Yeast Artificial Chromosome can accept an insert of about
0.5 - 3 Mb
System that controls the expression vector
lac operon regulatory system
