P7 IMMUNOLOGY Flashcards
most important pre-transplant test
Crossmatching test
mention the 3 pre-transplant test
-HLA-TYPING
- crossmatching
- PRA
Transplantation of cells, tissues or organs between sites within the same individual e.g. skin graft.
autograft transplantation
Transplantation of organs or tissues from a donor to a non-genetically identical individual of the same species
allograft transplantation
most common type of transplant
allograft transplantation
Transplantation of an organ or tissue between 2 different species
Xenograft
a cluster of genes important in
* Immune recognition
* Signalling between cells of the immune system
HLA complex /MHC
class 1 MHC are seen on which cells
all nucleated cells
class 2 MHC are seen on which cells
Mononuclear cells (lymphocytes & macrophages )
class 1 antigens
HLA A . B . Cw
class 2 antigens
HLA DR , DQ , DP
function of class 1 HLA molecules
Antigen recognition by a cytotoxic T- lymphocyte (CD8)
explain the immune response behind Organ Transplantation Rejection
The foreign(donor’s) class I HLA are recognized by the host (recipient’s) CD8 T- lymphocytes during REJECTION
explain the immune response behind GVHD
Donor T cells attack Patient’s (recipient’s)
tissue(different HLA class II Ags)
function of HLA typing
- Paternity testing
- Prior to Transplantation to select donor
- Disease association
the process of Amplification of specific targeted regions of DNA is known as
PCR
cross matching used for
Detection of preformed antidonor antibodies before transplantation
Advantages of FCXM over CDC
- Detects non complement mediated antibodies
- Faster turn around time
- Lower incidence of ambiguous results
- direct detection of IgG
