Microscopy - Cell Biology Flashcards
Centimetre to millimetre
X10
Millimetre to micrometre
X1000
Micrometre to Nanometre
X1000
Rank the units of measurement
- Centimetre
- Millimetre
- Micrometre
- Nanometre
How do you calculate magnification?
Magnification = Image size/actual size
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A M
What is magnification?
The degree to which the size of an image is larger than the object itself/ How many times bigger the size of the image is than the object
What is resolution?
Thee degree to which it’s possible to distinguish between 2 objects that are very close together
What is the diaphragm?
Controls amount of light that enters and exists
Benefits of light microscope:
- Have colour
- Cheaper
- Portable
- Can see live substances
Disadvantage of light microscope:
- Lower resolution and magnification in comparison to electron microscopes
Benefits of electron microscope:
- Higher magnification and resolution
Disadvantages of electron microscope:
- Expensive
- Too large
- Can’t see live specimens
- Black & white
What does the mirror do in light microscopes?
It reflects light into the lens then into the eye
How does an electron microscope work?
It uses a beam of electrons
What is a scanning electron microscope?
It lets you view specimens in 3D