Microscopy - Cell Biology Flashcards

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1
Q

Centimetre to millimetre

A

X10

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2
Q

Millimetre to micrometre

A

X1000

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3
Q

Micrometre to Nanometre

A

X1000

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4
Q

Rank the units of measurement

A
  1. Centimetre
  2. Millimetre
  3. Micrometre
  4. Nanometre
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5
Q

How do you calculate magnification?

A

Magnification = Image size/actual size

I

A M

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6
Q

What is magnification?

A

The degree to which the size of an image is larger than the object itself/ How many times bigger the size of the image is than the object

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7
Q

What is resolution?

A

Thee degree to which it’s possible to distinguish between 2 objects that are very close together

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8
Q

What is the diaphragm?

A

Controls amount of light that enters and exists

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9
Q

Benefits of light microscope:

A
  • Have colour
  • Cheaper
  • Portable
  • Can see live substances
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10
Q

Disadvantage of light microscope:

A
  • Lower resolution and magnification in comparison to electron microscopes
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11
Q

Benefits of electron microscope:

A
  • Higher magnification and resolution
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12
Q

Disadvantages of electron microscope:

A
  • Expensive
  • Too large
  • Can’t see live specimens
  • Black & white
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13
Q

What does the mirror do in light microscopes?

A

It reflects light into the lens then into the eye

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14
Q

How does an electron microscope work?

A

It uses a beam of electrons

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15
Q

What is a scanning electron microscope?

A

It lets you view specimens in 3D

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16
Q

Why do electron microscopes have a higher magnification and resolution?

A

Electron beams have a smaller wavelength than light beams.