Microscopy Flashcards

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1
Q

Many of the cell contents are…

A

colourless so they need to be stained to be seen.

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2
Q

The deeply staining material in the nucleus is…

A

chromatin, which is a mass of loosely coiled threads.

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3
Q

Chromatin collects together…

A

to form visible separate chromosomes during nuclear division.

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4
Q

The nucleolus is made of…

A

loops of DNA from several chromosomes. It is even more deeply staining area than chromatin.

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5
Q

The number of nucleoli is…

A

variable, one to five being common in mammals.

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6
Q

Organelle

A

Functionally and structurally distinct part of a cell. Often surrounded by membranes.

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7
Q

Why are many organelles surrounded by membranes?

A

So that their activities can be separated from the surrounding cytoplasm. This is called compartmentalisation.

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8
Q

Compartmentalisation is essential for a strucutre…

A

as complex as an animal or plant cell to work efficiently.

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9
Q

Division of labour means that…

A

work is shared between differently specialised organelles.

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10
Q

The most numerous organelles seen with a light microscope are usually…

A

mitochondria. They can move, change shape and divide.

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11
Q

Golgi apparatus is a part…

A

of complex internal sorting and distribution system within the cell.

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12
Q

Plasmodesmata

A

Fine strands of cytoplasm which pass through pore-like structures in their walls.

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13
Q

Phagocytic vacuoles

A

Small temporary vacuoles that can be found in animal cells.

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14
Q

The plant vacuole is surrounded by a membrane called…

A

tonoplast, which controls exchange between cytoplasm and the vacuole.

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15
Q

The fluid in the plant vacuole is…

A

a solution of pigments, enzymes, sugars, waste products, mineral salts, oxygen and CO2.

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16
Q

The plant vacuole helps to…

A

regulate the osmotic properties of cells as well as having a wide range of other functions. E.g. red pigment of beetroot, may be located in vacuoles.

17
Q

Granum

A

Located in the chloroplast. They contain chlorophyll. Can be seen with a light microscope.

18
Q

Cell walls are…

A

freely permeable, unlike the cell surface membrane.

19
Q

Smallest structure visible with a human eye is…

A

50-100 micro meters.

20
Q

Magnification

A

The number of times larger an image is than in the real size of the object.

21
Q

Magnification formula

A

Observed size/actual size

22
Q

Resolution

A

The ability to distinguish between two separate points. If the two points cannot be resolved, they will be seen as one point.

23
Q

The maximum resolution of a light microscope is…

A

200 nm.

24
Q

The length of visible light waves is…

A

from 400nm (violet light) to 700nm (red light).

25
Q

The greater the energy…

A

the shorter the wavelength.

26
Q

The limit of resolution is about…

A

one half the wavelength of the radiation used to view the spiecemen.

27
Q

Maximum useful magnification of a light microscope is…

A

1500x

28
Q

If the object is transparent…

A

it must be stained, otherwise, the waves would pass through it.

29
Q

Why are electrons used as a source of radiation?

A
  • wavelength is extremely short

- because they are negatively charged they can be focused easily using electromagnets

30
Q

Resolution of an electron microscope is…

A

0.5 nm.

31
Q

TEM

A

Transmission electron microscope. Only electrons that pass through the specimen are seen. Allowing us to see internal structures.

32
Q

SEM

A

Scanning electron microscope. Only the reflected beam is observed. This allows us to see the surfaces. 3D appearance is achieved.

33
Q

Resolution of SEM

A

3nm-20nm

34
Q

To see the beam of electrons…

A

the fluorescent screen must be used(very expensive).