Enzyme Affinities Flashcards
Affinity is…
Turnover rate of an enzyme(speed)
When V-max, enzyme is…
Saturated with substrate at the maximum rate
Michaelis-Menten constant
(Km) is used to compare the affinity of different enzymes for their substrates
How to find the Km?
Vmax / 2 =to substrate concentration for Km
The lower the Km…
The higher the affinity of an enzyme.
An enzyme with low Km:
- has a high affinity to its substrate
- needs a lower concentration of substrate to reach V max than enzyme with a high Km
An enzyme with low Km is more likely to be…
Saturated with substrate in the normal conditions of substrate within a cell, so variations in substrate will have less effect on the formation of product
Intercellular enzymes
Remain to function intracellularly.
Enzymes are produced within…
Cells.
Extracellular enzymes are…
Released to act extracellularly e.g. digestive enzymes
The importance of Vmax and Km commercially important enzymes…
the performance of the same enzyme can be compared from different organisms
(The importance of Vmax and Km) helps scientist to make…
models of biochemical pathways (helps to predict reaction)
(The importance of Vmax and Km) the calculations involved can be…
Applied to other fields of biochemistry such as antigen-antibody binding
(The importance of Vmax and Km) knowing the Km means the proportion…
Of active sites occupied by substrate molecules can be calculated for any substrate concentration.
