Factors affecting the enzyme activity Flashcards
Enzyme activity can be affected by:
- enzyme concentration
- substrate concentration
- temperature
- pH
The more enzyme present…
the more active sites available.
As long as there is plenty of substrates available…
the initial rate of reaction increases with enzyme concentration. It is directly proportional.
If the rate of reaction stops increasing with higher enzyme concentration…
all substrate was used to make the enzyme-substrate complex.
As the substrate concentration increases…
the rate of reaction also increases.
The more molecules of the substrate around…
the more often an enzyme’s active site can bind with one.
If the enzyme reaches its maximum…
possible rate=Vmax (max. velocity) all active sites are occupied.
At low-temperature, the reaction takes place very slowly as…
the molecules move more slowly.
At higher temperature molecules move…
faster so they collide with the active side more often.
At higher temperature more energy is involved so…
it is easier for bonds to be formed.
If the temperature is too high…
bonds between enzyme break and enzyme denaturates.
Optimum temperature is…
the temperature at which a maximum rate of reaction of enzyme takes place. The temperature is not a limiting factor anymore.
Most of the enzymes work best at what ph?
Around 7
Too different pH can cause…
Denaturation
The lower the pH the higher…
The hydrogen ion concentration.
