Lecture 43: Muscle tissue with HISTO Flashcards
functions of muscle tissue
- movement 2. maintenance of posture 3. joint stabilization 4. heat generation
special functional characteristics of muscle
- contractility 2. excitability 3. extensibility 4. elastictity
contractility
only one action, to shorten. shortening generates pulling force
excitability
nerve fibers cause electrical impulse to travel
extensibility
stretch with contraction of an opposing muscle
elasticity
recoils passively after being stretch
what percent of body weight to skeletal muscle make up
40%
what kind of fibers in skeletal muscle
multinucleate cells (embryonic cells fuse)
type of contractions in skeletal muscle
voluntary
where is cardiac muscle found
in the wall of the heart
where is smooth muscle found
walls of hollow organs
what do skeletal muscles attach
one bone to another and cross at least one moveable join
origin and insertion
origin is the less moveable attachment, insertion gets pulled towards the origin (usually one bone moves and the other remains fixed)
convention of location of origin and insertion in muscles of the limb
origin lies proximal to insertion
biarticular muscles
span two or more joints (also called multijoint) and cause movements at two joints
direct attachment
attachment is so short that it appears to attach directly to bone
indirect attachment
connective tissue extends well beyond the muscle (more common) ex tendons
can skeletal muscle regenerate
minimally
sarcolema
plasmalemma or muscle plasma membrane
sarcoplasmic reticulum
muscle cell ER
sarcoplasm
muscle cell cytoplasm
motor unit
1 motor nerve and all the muscle fibers it innervates
muscle cells are called
fibers
characteristics of muscle cells
multinucleated, peripherally located, surrounded by delicate connective tissue (endomysium)
structure of muscle cell
fibers surrounded by endomysium and grouped into bundles called fascicles surrounded by perimysium. bundles of fasicles make up a muscle and are separated by epimysium
muscle fibers are made up of
many myofibrils whcih are bundles of myofilaments (thin actin, thick myosin) organized into sarcomeres
what are myofibrils surrounded by
sarcoplasmic reticulum and composed of sarcomeres (z to z line)
sarcomere is
basic contractile unit of myofiber
subdivisions of sarcomere
A band: dark band containing actin and myosin I band: light band with only actin Z line: binds the I band, anchors actin, defines borders of sarcomeres H band: light band, bisects the A band, contains only myosin M line: dark line, bisects H band, anchors myosin filaments
three types of filaments in myofibrils
- thick (myosin) 2. thin (actin) 3. elastic (titin)
what provides the driving force for movement
Myosin II head as ATP drives movement of myosin along actin
what is on the actin filaments that allows it to bind myosin
troponin (C, , and T) and tropomyosin which have myosin binding sites
what is alpha actinin
it is incoporated into the Z disks and attaches actin to the disks
SR is comparable to
Smooth ER. tubules surround the myofibrils
terminal cisternae
cross channels that surround myofibrils. store Ca and release when muscle is stimulated to contract by action potentials.
what are t tubules continuous with
sarcolemma so whole muscle (including deep parts) contract simultaneously
chemical cascade causing muscle contraction
- neutrotransmitters from motor end plate cause depolarization of sarcolemma (including t tubules) 2. depolarization extends to membranes of SR 3. sudden release of Ca in sarcoplasm 4. Ca binds to troponin C which seperates troponin I from actin and exposes myosin binding site *Contraction begins
how does calcium get removed from contracting muscle cell
actively pumped back into SR
what is the triad in muscle cells
t tubule and 2 terminal cisternae
physical explanation of muscle contraction
- myosin head (high energy with ADP and Pi) cross bridge attaches to actin myofilament 2. Myosin head pivots and bends as it bends and pulls on actin filament sliding towards M line (ADP and Pi released to ATP) 3. myosin head gets a new ATP attached and it detaches from actin 4. ATP hydrolyzed to ADP and Pi and this cocks the myosin head back to high energy configuration
what do satellite cells do
help with muscle generation
myasthenia gravis
autoimmune disease in which antibodies are produced against acetylcholine receptors so acetylcholine cant bind and there isnt normal muscle interaction. get muscle weakness.
cardiac muscle characteristics
- fibers branch 2. central nuclei (1-2) 3. intercalated disks where cells join 4. no satellite cells 5. t tubules at Z lines 6. cross striations
what do bundles in cardiac muscle form
thick myocardium
cardiac muscle cells
single cells (NOT fibers)
fiber in cardiac muscle refers to
long row of joined cardiac muscle cells (fibers form syncitium)
inherent rhythmicity
each cell beats seperately without any stimulation
diad
in cardiac muscle cell, terminal cisternae with t tubule
where are t tubules found in cardiac vs skeletal muslce
cardiac: level of Z disk skeletal: A-I junction
6 major locations of smooth muscle
- inside the eye 2. walls of vessels 3. respiratory tubes 4. digestive tubes 5. urinary organs 6. reproductive organs
characteristics of smooth muscle
- spindle shaped cells 2. one central nucleus (contour can be irregular during contraction) 3. grouped into sheets that often run perpendicular to each other 4. peristalsis 5. no striations because no sarcomeres 6. contractions are slow, sustained, and resistant to fatigue 7. does not require nervous signal, can be stimulated by stretching or hormones
what is analogous to z disks in smooth muscle
alpha-actinin rich “cytoplasmic densities” associated with cell membranes
how do smooth muscles contract
- in the absence of t tubules, calcium is brought into cells by endocytic vacuoles (calveolae) 2. calcium binds with calmodulin 3. Ca-calmodulin complex – myosin light chain kinase phosphorylates 4. resulting conformational change exposes actin binding site of myosin and you get contraction
where are dense bodies found
beneath plasma membrane and in the cytoplasm and contain site of insertion of actin filaments
is there myosin filaments in smooth muscle
yes!
regenerative capacity of the three types of muscle
- cardiac : virtually no regenerative capacity in adults 2. skeletal: nuclei cannot undergo mitosis but tissue can undergo limited regeneration via satellite cells 3. smooth: has regenerative capacity (ex: enlargement of uterus during pregnancy)
actin genes
at least four 1. cardiac actin 2. skeletal actin 3. vascular smooth muscle actin 4. visceral smooth muscle actin *changes in genes results in myopathies
how can we distinguish myopathies from neuropathies
histo!!!

cardiac muscle
- macula adherens
- gap junction
- fascia adherens

cardiac muscle

cardiac muscle

dense bodies smooth muscle

skeletal muscle

cardiac muscle (high mag)

smooth muscle (low mag)

neuromuscular junction

skeletal muscle
green = A bands
black = I bands

skeletal muscle with tendon attachment, see bony spicules on either side

skeletal muscle

muscle sarcomere
black = z line
grey in middle = m line

skeletal muscle
green is endomysium
black is perimysium

green is perimysium, red is muscle fibers

skeletal muscle with RBCs around

smooth muscle

smooth muscle surface calveolae, t tubules

smooth muscle

neuromuscular junction

skeletal muscle triad

skeletal muscle
SR = sarocplasmic reticulum
M = mitochondria