Lecture 4 Flashcards

1
Q
A

bree and becca

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2
Q

H&E stands for

A

hematoxylin and eosin

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3
Q

hematoxylin

A

combines with Al+++ ions to form a + charged complex

BASOPHILIC so stains basic structures with - charge

Blue to purple in color

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4
Q

Eosin

A

Orange color and negatively charged

Acid stain – acidophilic and stands acid structures

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5
Q

Euchromatin

A

DNA dispersed, acitve transcription of RNA, lightly stained but basophilic

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6
Q

heterochromatin

A

DNA is condensed, inactive transcription, well stained, basophilic

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7
Q

nucleolus

A

RNA, ribosome production. basophilic

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8
Q

rough ER

A

ribosomes, RNA, secretory protein synthesis, Basophilic

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9
Q

free ribosomes

A

cystolic protein synthesis

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10
Q

smooth ER

A

lipid synthesis, drug and alcohol metabolism, glycogen strorage

ACIDOPHILIC

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11
Q

golgi

A

carb synthesis, secretion (lightly stained but acidophilic)

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12
Q

mitochondria

A

ATP production, acdiophilic

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13
Q

lysosomes

A

digestion, lightly stained but acidophilic

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14
Q

microtubules

A

movement of molecules, mitosis, motility

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15
Q

filaments

A

support, contraction, acidophilic

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16
Q

lipid droplets

17
Q

glycogen

A

energy, stained lightly

18
Q

what are the RER and golgi involved in

A

synthesis of antibodies - glycoproteins

golgi makes carbs

19
Q

Feulgen reaction

A

Mild acid hydrolysis to cleave nucleotide from deoxyribose – makes an aldehyde which can react with the Schiff (leucofuchsin) agent and results in a pink color

20
Q

PAS reaction

A

periodic acid schiff reaction

Detects carb rich macromolecules, GAGs, glycoproteins, glycogen

21
Q

GAGs

A

glycosaminoglycans

22
Q

chemical basis of PAS reaction

A

Periodic acid oxidizes free hydroxyl on two adjacent C atoms in the hexose residue to form 2 aldehydes – these react with Schiff to make magenta product

23
Q

ex of what PAS stains deep red

A

mucus of goblet cells and glycoprotein of glycocalyc

Basement membrane (because heparan sulfate = GAG)

24
Q

what is glycogen stained by

A

PAS method

25
metachromasia
staining rxn in which the cell components are stained a different color from the dye itself
26
toluidine blue
metachromatic positively charged dye which is deep ble in color stains heparin in mast cells
27
examples of lipid stains
sudan IV (fat) osmium tetroxide (OsO4) stains myelin (lecithin, sphingomyelin)
28
extracellular matrix composed of
elastic fibers and collagen fibers
29
elastic fibers stain
acidophilic in H&E black with resorcin fuchsin
30
collagen fibers stain
acidophilic in H&E blue with mallory trichrome (type I&III) black with silver stain (reticular/type III)
31
marker for lysosomes
acid phosphatase
32
stain for acid phosphatase
azo dye (red)
33
oxidative enzyme of CAC
succinate dehydrogenase, allows staining of mitochondria
34
staining of succinate dehydrogenase
tetrazolium method, stain ends up being formazan, black
35
what marks antibodies
fluorescent compound or peroxidase
36
immunoperoxidase stains
insulin in islet of langerhan in pancreas