Lecture 4 Flashcards
bree and becca
H&E stands for
hematoxylin and eosin
hematoxylin
combines with Al+++ ions to form a + charged complex
BASOPHILIC so stains basic structures with - charge
Blue to purple in color
Eosin
Orange color and negatively charged
Acid stain – acidophilic and stands acid structures
Euchromatin
DNA dispersed, acitve transcription of RNA, lightly stained but basophilic
heterochromatin
DNA is condensed, inactive transcription, well stained, basophilic
nucleolus
RNA, ribosome production. basophilic
rough ER
ribosomes, RNA, secretory protein synthesis, Basophilic
free ribosomes
cystolic protein synthesis
smooth ER
lipid synthesis, drug and alcohol metabolism, glycogen strorage
ACIDOPHILIC
golgi
carb synthesis, secretion (lightly stained but acidophilic)
mitochondria
ATP production, acdiophilic
lysosomes
digestion, lightly stained but acidophilic
microtubules
movement of molecules, mitosis, motility
filaments
support, contraction, acidophilic
lipid droplets
energy
glycogen
energy, stained lightly
what are the RER and golgi involved in
synthesis of antibodies - glycoproteins
golgi makes carbs
Feulgen reaction
Mild acid hydrolysis to cleave nucleotide from deoxyribose – makes an aldehyde which can react with the Schiff (leucofuchsin) agent and results in a pink color
PAS reaction
periodic acid schiff reaction
Detects carb rich macromolecules, GAGs, glycoproteins, glycogen
GAGs
glycosaminoglycans
chemical basis of PAS reaction
Periodic acid oxidizes free hydroxyl on two adjacent C atoms in the hexose residue to form 2 aldehydes – these react with Schiff to make magenta product
ex of what PAS stains deep red
mucus of goblet cells and glycoprotein of glycocalyc
Basement membrane (because heparan sulfate = GAG)
what is glycogen stained by
PAS method
metachromasia
staining rxn in which the cell components are stained a different color from the dye itself
toluidine blue
metachromatic
positively charged dye which is deep ble in color
stains heparin in mast cells
examples of lipid stains
sudan IV (fat)
osmium tetroxide (OsO4) stains myelin (lecithin, sphingomyelin)
extracellular matrix composed of
elastic fibers and collagen fibers
elastic fibers stain
acidophilic in H&E
black with resorcin fuchsin
collagen fibers stain
acidophilic in H&E
blue with mallory trichrome (type I&III)
black with silver stain (reticular/type III)
marker for lysosomes
acid phosphatase
stain for acid phosphatase
azo dye (red)
oxidative enzyme of CAC
succinate dehydrogenase, allows staining of mitochondria
staining of succinate dehydrogenase
tetrazolium method, stain ends up being formazan, black
what marks antibodies
fluorescent compound or peroxidase
immunoperoxidase stains
insulin in islet of langerhan in pancreas
