Lecture 4

Question 1

Answer 1

bree and becca

Question 2

H&E stands for

Answer 2

hematoxylin and eosin

Question 3

hematoxylin

Answer 3

combines with Al+++ ions to form a + charged complex

BASOPHILIC so stains basic structures with - charge

Blue to purple in color

Question 4

Eosin

Answer 4

Orange color and negatively charged

Acid stain – acidophilic and stands acid structures

Question 5

Euchromatin

Answer 5

DNA dispersed, acitve transcription of RNA, lightly stained but basophilic

Question 6

heterochromatin

Answer 6

DNA is condensed, inactive transcription, well stained, basophilic

Question 7

nucleolus

Answer 7

RNA, ribosome production. basophilic

Question 8

rough ER

Answer 8

ribosomes, RNA, secretory protein synthesis, Basophilic

Question 9

free ribosomes

Answer 9

cystolic protein synthesis

Question 10

smooth ER

Answer 10

lipid synthesis, drug and alcohol metabolism, glycogen strorage

ACIDOPHILIC

Question 11

golgi

Answer 11

carb synthesis, secretion (lightly stained but acidophilic)

Question 12

mitochondria

Answer 12

ATP production, acdiophilic

Question 13

lysosomes

Answer 13

digestion, lightly stained but acidophilic

Question 14

microtubules

Answer 14

movement of molecules, mitosis, motility

Question 15

filaments

Answer 15

support, contraction, acidophilic

Question 16

lipid droplets

Answer 16

energy

Question 17

glycogen

Answer 17

energy, stained lightly

Question 18

what are the RER and golgi involved in

Answer 18

synthesis of antibodies - glycoproteins

golgi makes carbs

Question 19

Feulgen reaction

Answer 19

Mild acid hydrolysis to cleave nucleotide from deoxyribose – makes an aldehyde which can react with the Schiff (leucofuchsin) agent and results in a pink color

Question 20

PAS reaction

Answer 20

periodic acid schiff reaction

Detects carb rich macromolecules, GAGs, glycoproteins, glycogen

Question 21

GAGs

Answer 21

glycosaminoglycans

Question 22

chemical basis of PAS reaction

Answer 22

Periodic acid oxidizes free hydroxyl on two adjacent C atoms in the hexose residue to form 2 aldehydes – these react with Schiff to make magenta product

Question 23

ex of what PAS stains deep red

Answer 23

mucus of goblet cells and glycoprotein of glycocalyc

Basement membrane (because heparan sulfate = GAG)

Question 24

what is glycogen stained by

Answer 24

PAS method

Question 25

metachromasia

Answer 25

staining rxn in which the cell components are stained a different color from the dye itself

Question 26

toluidine blue

Answer 26

metachromatic

positively charged dye which is deep ble in color

stains heparin in mast cells

Question 27

examples of lipid stains

Answer 27

sudan IV (fat)

osmium tetroxide (OsO4) stains myelin (lecithin, sphingomyelin)

Question 28

extracellular matrix composed of

Answer 28

elastic fibers and collagen fibers

Question 29

elastic fibers stain

Answer 29

acidophilic in H&E

black with resorcin fuchsin

Question 30

collagen fibers stain

Answer 30

acidophilic in H&E

blue with mallory trichrome (type I&III)

black with silver stain (reticular/type III)

Question 31

marker for lysosomes

Answer 31

acid phosphatase

Question 32

stain for acid phosphatase

Answer 32

azo dye (red)

Question 33

oxidative enzyme of CAC

Answer 33

succinate dehydrogenase, allows staining of mitochondria

Question 34

staining of succinate dehydrogenase

Answer 34

tetrazolium method, stain ends up being formazan, black

Question 35

what marks antibodies

Answer 35

fluorescent compound or peroxidase

Question 36

immunoperoxidase stains

Answer 36

insulin in islet of langerhan in pancreas