Lecture 4 Flashcards
bree and becca
H&E stands for
hematoxylin and eosin
hematoxylin
combines with Al+++ ions to form a + charged complex
BASOPHILIC so stains basic structures with - charge
Blue to purple in color
Eosin
Orange color and negatively charged
Acid stain – acidophilic and stands acid structures
Euchromatin
DNA dispersed, acitve transcription of RNA, lightly stained but basophilic
heterochromatin
DNA is condensed, inactive transcription, well stained, basophilic
nucleolus
RNA, ribosome production. basophilic
rough ER
ribosomes, RNA, secretory protein synthesis, Basophilic
free ribosomes
cystolic protein synthesis
smooth ER
lipid synthesis, drug and alcohol metabolism, glycogen strorage
ACIDOPHILIC
golgi
carb synthesis, secretion (lightly stained but acidophilic)
mitochondria
ATP production, acdiophilic
lysosomes
digestion, lightly stained but acidophilic
microtubules
movement of molecules, mitosis, motility
filaments
support, contraction, acidophilic
lipid droplets
energy
glycogen
energy, stained lightly
what are the RER and golgi involved in
synthesis of antibodies - glycoproteins
golgi makes carbs
Feulgen reaction
Mild acid hydrolysis to cleave nucleotide from deoxyribose – makes an aldehyde which can react with the Schiff (leucofuchsin) agent and results in a pink color
PAS reaction
periodic acid schiff reaction
Detects carb rich macromolecules, GAGs, glycoproteins, glycogen
GAGs
glycosaminoglycans
chemical basis of PAS reaction
Periodic acid oxidizes free hydroxyl on two adjacent C atoms in the hexose residue to form 2 aldehydes – these react with Schiff to make magenta product
ex of what PAS stains deep red
mucus of goblet cells and glycoprotein of glycocalyc
Basement membrane (because heparan sulfate = GAG)
what is glycogen stained by
PAS method