lecture 13 part 2

Question 1

where do substrates bind

Answer 1

at the active site of the enzyme

Question 2

enzyme-substrate binding is very _____

Answer 2

specific

Question 3

do enzymes catalyze one biochem rxn over and over or different biochem rxns

Answer 3

one biochem rxns over and over

Question 4

what is induced fit between an enzyme and its usbtrate
-what does it bring into position

Answer 4

a tightening of the enzyme’s active site around the substrate
-brings a chem group into position to enhance the enzymes ability to catalyze the reaction

Question 5

tight enzyme substrate complex

Answer 5

puts the subtrate into its high energy transiion state allowing catalysis and formation of products

Question 6

how can a tight fit between substrate impact Ea

Answer 6

lower the Ea of a biochem rxn by
-orienting substances correctly
-straining substrate bonds
-providing a favirable microenvironment that facilitates making/breaking of covalent bonds
-covalently bonding to substrate

Question 7

enzyme kinetics

Answer 7

study of enzyem catalyzed rxn rates and the conditions which affect them

Question 8

Vmax

Answer 8

max rate of rxn/how fast it can go

Question 9

Km

Answer 9

subatrte [] required for the rxn rate to reach 1/2 Vmax

Question 10

what is Vmax rate limited by

Answer 10

substrate []

Question 11

Km rate is dependent on

Answer 11

substrate []

Question 12

when substrate is in excess, what happens to rxn rate when more enzymes are added

Answer 12

increases rxn rate

Question 13

what is Km related to

Answer 13

affinity of the enzyme for the substrate

Question 14

what is Km not equal to

Answer 14

affinity of the enzymes for the substrate

Question 15

what does Km depend on

Answer 15

structure of enzyme + substrate

Question 16

Km is independent of what

Answer 16

substrate [] and enzyme []

Question 17

what does smaller Km need less of

Answer 17

less substrate/small [] to reach 1/2 Vmax

Question 18

smaller Km =

Answer 18

larger affinity

Question 19

Km indicates

Answer 19

affinity (attraction) between enzyme + substrate

Question 20

what are most important non covalent bonds

Answer 20

-h bonds
-ioic bonds
-van der waals
-electrostatic interactions

Question 21

oxidize

Answer 21

lose e-

Question 22

reduction

Answer 22

gain e-

Question 23

if cells need to inc rate of rxn it will express the gene of higher levels through what

Answer 23

protein synthesis/transcription (upregulate trasncription)