LEC 7 - PHOSPHOROUS Flashcards
It is an important constituent in nucleic acid, phospholipid
and phosphoproteins,
PHOSPHORUS
phosphorus’s distribution
80% in the bone
20% in soft tissues
1% in plasma
phosphorous concentration is inversely related to
calcium
if the calcium is maximally absorbed in duodenum, what about phosphorus?
jejunum
role of phsphate in glucose and insulin processing
essential for the insulin-mediated entry of glucose into the cells by a process involving PHOSPHORYLATION of the glucose and the co entry of K+
in PHOSPHORUS we are determining __
phosphate
inorganic phosphorous is called
phosphates
Phosphorous
It forms high energy compounds such as ______and is involved in intermediary metabolism and
various enzyme systems
ATP
nucleic acid (dna and rna)
enzyme cofactors (NADPH)
lipids (phospholipids)
most phosphate in serum is in what form
inorganic form
It is essential for muscle contractility, neurologic function,
and electrolyte transport and oxygen-carrying by
hemoglobin.
PHOSPHORUS
is the primary factor that increase phosphate
Vitamin D
relationship of PTH to phosphate
inversely - PTH want calcium
hyperphosphatemia usual cause
Decrease renal excretion in acute and chronic renal failure
Increase intake with excessive oral, rectal, intravenous
administration.
hyper or hypo?
hyperphosphatemia
Increase extracellular load due to transcellular shift in acidosis in phosphate
hyperphosphatemia
Secondary to over medication with Vitamin D and
production of Vitamin by granulomatous tissue.
effect to phosphate
hyperphosphatemia
phosphate is usually seen in children ___
drinking cow’s milk
phosphate is usually seen in adult ___
taking laxatives
patients with lymphoblastic leukemia
hyperphosphatemia or hypo?
hyperphosphatemia
Hypoparathyroidism
hyperphosphatemia or hypo?
hyper
t is a condition characterized by a serum phosphorus
concentration below the lower limit of the reference interval
HYPOPHOSPHATEMIA
HYPOPHOSPHATEMIA can be seen in
o Alcohol abuse
o Intestinal loss due to vomiting, diarrhea, and use of phosphate binding antacids
o Induced by a shift of phosphorus from extracellular fluid into cells.
decreased intestinal absorption, as with vitamin D
deficiency or antacid use and steatorrhea
hyperphosphatemia or hypo?
hypo
Most of the current methods for phosphorus determination
involve the formation of an ____
ammonium
phosphomolybdate complex
an ammonium
phosphomolybdate complex.
o This colorless complex can be measured by ultraviolet
absorption at _____ nm
340
- Most of the current methods for phosphorus determination
involve the formation of an ammonium
phosphomolybdate complex.
o This colorless complex can be measured by ultraviolet
absorption at 340 nm or can be reduced to form molybdenum blue, a stable blue chromophore, which
is read between _____ nm.
600 and 700
Reaction of phosphate with ammonium molybdate (_____)
FISKE SUBBAROW
REFERENCE INTERVAL for phosphorous
Inserts
* Adult: 2.8-4.5 mg/dL (0.89-1.44mmol/L)
* Children: 4.0-7.0mg/dL (1.29-2.26mmol/L)
the type of phosphate that is the principal anion within cells
organic phosphate
the type of phosphate that is pary pf the blood buffer system
inorganic phosphate - kaya ang highest type of phosphate na makikita sa serum is si inorganic kasi pang buffer siya
free or unbound form of phosphate percentage
55%
complexed with ions percentage of phosphate
35%
protein bound percentage of phospahte
10%
role of PTH in phosphate
secretes phosphate - decrease
role of growth hormone in phosphate
reabsorption - increase
do we need to have the patient undergo fasting for phosphate determination?
yes, a high CHO can result in decreased levels
what is the time (circadian rhythm) when phosphate has high production
late morning
low levels in the evening
effect of physical activty to phosphate
increase
the most commonly used method to measure serum inorganic phosphate
fiske-subbarow method
most common reagent in fiske subbarow
PICTOL (amino naphthol sulfonic acid )
end product of fiske subbarow
ammonium molybdate complex
the reduced form of the end product yield what color
blue
major cause of hypophosphatemia
transcellular shift
most common cause of hypophosphatemia
alcohol abuse
