LAB3- ALT

Question 1

The major source of ALT is of

Answer 1

hepatic origiN

Question 2

Elevated serum levels are found in .

Answer 2

hepatitis,
cirrhosis,
and obstructive
jaundice

Question 3

UV methods for ALT determination were first developed by ___ and ____ in ___

Answer 3

Wroblewski and LaDue in 1956

Question 4

UV methods for ALT determination

The method was based on the oxidation of

Answer 4

NADH by lactate dehydrogenase (LDH)

Question 5

In 1980, the _____ recommended a reference procedure for the measurements of ALT based on the Wroblewski and LaDue procedure

Answer 5

International Federation of Clinical Chemistry

Question 6

The Stanbio ALT reagent is based on a modified formulation of the

Answer 6

IFCC 3
and
Bergmeyer4 methods.

Question 7

first reaction in alt determination

Answer 7

L-Alanine + 2- Oxoglutarate

catalyze by enzyme ALT

Pyruvate + L-Glutamate

Question 8

2nd reaction in ALT determination

Answer 8

Pyruvate + NADH + H+

catalyze by LDH

D-Lactate + NAD+ + H2
O

Question 9

The rate of decrease in absorbance at ___ nm is directly proportional to ALT activity.

Answer 9

340

Question 10

Working Reagent in the ratio of

Answer 10

5 parts Buffer (R1)
to 1 part Enzyme (R2),

Question 11

Reagents are stable until the ____ on their respective labels, when properly stored at 2-8°C and
protected from light

Answer 11

expiration date

Question 12

storing of reagents in 2-8 * C wll last until?

Answer 12

expiration date

Question 13

Reagents should appear

Answer 13

clear and colorless.

Question 14

what to do if either appears cloudy or contains particulate matter

Answer 14

Discard

Question 15

The Working Reagent is stable for how many weeks at 2-8°C

Answer 15

4 weeks

Question 16

the working reagent is stable for how many days in room temperature
15-30*C

Answer 16

5 days

Question 17

The Working Reagent should be discarded if the initial
absorbance, read against distilled water at 340 nm, is below

Answer 17

0.800

Question 18

__ is the specimen of choice

Answer 18

Non-hemolyzed serum

Question 19

Whenever possible
specimens should be separated and analyzed on the day of collection

true or falset

Answer 19

true

Question 20

in storing 4oC, about how many percent of ALT is lost for 3days

Answer 20

10%

Question 21

in storing 25oC, about how many percent of ALT is lost for 1 days

Answer 21

10%

Question 22

:Hemolysis must be avoided as the concentration
of ALT in red cells is roughly __ that of serum

Answer 22

5 times

Question 23

Bilirubin levels up to

Answer 23

40 mg/dL

Question 24

triglyceride levels up to

Answer 24

2000 mg/dl

Question 25

how many minutes we do need to pre warm the working reagent before adding the 0.10 ml of serum

Answer 25

3 mns

Question 26

factor for computation

Answer 26

multiple yo 1768

Question 27

Normal Range:

Answer 27

3 - 35 U/L (37°C)

Question 28

If the ∆A/min. is greater than 0.16, what should we do

Answer 28

dilute 1 part sample with 9 parts
isotonic saline and re-assay. Multiply the result by 10. A