LAB3- ALT Flashcards
The major source of ALT is of
hepatic origiN
Elevated serum levels are found in .
hepatitis,
cirrhosis,
and obstructive
jaundice
UV methods for ALT determination were first developed by ___ and ____ in ___
Wroblewski and LaDue in 1956
UV methods for ALT determination
The method was based on the oxidation of
NADH by lactate dehydrogenase (LDH)
In 1980, the _____ recommended a reference procedure for the measurements of ALT based on the Wroblewski and LaDue procedure
International Federation of Clinical Chemistry
The Stanbio ALT reagent is based on a modified formulation of the
IFCC 3
and
Bergmeyer4 methods.
first reaction in alt determination
L-Alanine + 2- Oxoglutarate
catalyze by enzyme ALT
Pyruvate + L-Glutamate
2nd reaction in ALT determination
Pyruvate + NADH + H+
catalyze by LDH
D-Lactate + NAD+ + H2
O
The rate of decrease in absorbance at ___ nm is directly proportional to ALT activity.
340
Working Reagent in the ratio of
5 parts Buffer (R1)
to 1 part Enzyme (R2),
Reagents are stable until the ____ on their respective labels, when properly stored at 2-8°C and
protected from light
expiration date
storing of reagents in 2-8 * C wll last until?
expiration date
Reagents should appear
clear and colorless.
what to do if either appears cloudy or contains particulate matter
Discard
The Working Reagent is stable for how many weeks at 2-8°C
4 weeks
the working reagent is stable for how many days in room temperature
15-30*C
5 days
The Working Reagent should be discarded if the initial
absorbance, read against distilled water at 340 nm, is below
0.800
__ is the specimen of choice
Non-hemolyzed serum
Whenever possible
specimens should be separated and analyzed on the day of collection
true or falset
true
in storing 4oC, about how many percent of ALT is lost for 3days
10%
in storing 25oC, about how many percent of ALT is lost for 1 days
10%
:Hemolysis must be avoided as the concentration
of ALT in red cells is roughly __ that of serum
5 times
Bilirubin levels up to
40 mg/dL
triglyceride levels up to
2000 mg/dl
how many minutes we do need to pre warm the working reagent before adding the 0.10 ml of serum
3 mns
factor for computation
multiple yo 1768
Normal Range:
3 - 35 U/L (37°C)
If the ∆A/min. is greater than 0.16, what should we do
dilute 1 part sample with 9 parts
isotonic saline and re-assay. Multiply the result by 10. A
