LAB2 - AST Flashcards
is one of several enzymes that catalyze the exchange of amino acids and oxo groupds between alpha amino acids and alpha oxo acids
aspartate aminotransferase
ast is widely distributed throughout the body tissues with significant amounts in the
heart or liver
lesser amounts of AST are found in
skeletal muscle, kidney, pancreas, spleen, lungs, ad brain.
in myocardial infarction, serum AST may begin to rise within ___ after onset,
6-8 hours
in myocardial infarction, serum AST may begin to rise within 6-8 hours after onset, and will peak _____
within 2 days
in what day will AST go back to normal once MI occured
on fourth to fifth day of post infarction
coditions we can found increase serum AST
hepatitis, liver necrosis, cirrhosis, and liver metastasis
he/she first reported a kinetic method for measuring AST activity in serum
KARMEN
Subsequently, karmen’s kinetic method of measuring AST was been modified by
Bergmeyer et. al.
the first reaction in AST determination
L-aspartate + 2-oxoglutarate
catalyze by AST
L- glutamate + oxalacetate
2nd reaction in AST determination
oxalacetate + NADH + H
catalyze by malate dehydrogenase
L-malate + NAD
AST activity is determined by measuring the rate of oxidation of NADH at ____ nm
340
lactate dehydrogenase is inlcuded in the reagent to convert ______ in the sample to lactate
endogenous pyruvate
reagents are stable until the expiration date on their respective labels if stored in what temperature
2-8 * C
appearance of reagent
clear and colorless
