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CCHM 2 > LAB 6 - chloride > Flashcards

LAB 6 - chloride Flashcards

1
Q

classical methods for chloride determination have been based on the combination of this ion with either __ or ____

A

ion + silver
ion + mercury

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2
Q

classical methods for chloride determination have been based on the combination of this ion with either silver or mercury to form ___

A

undissociated chloride compound

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3
Q

most popular for the estimation of chloride in body fluids since 1941 gas has been the technique ____

A

titrametric technique of shales and shales

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4
Q

titrametric technique of shales and shales uses a standardized solution of __

A

mercuric nitrate and diphenylcarbazone

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5
Q

purpose of diphenylcarbazone in the titrametric technique of shales and shales

A

indicator

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6
Q

the colorimetric procedure (which we did in lab) is based on the water analysis scheme of ___

A

Zall et.al

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7
Q

the colorimetric procedure (which we did in lab) is based on the water analysis scheme of Zall et.al which was later adapted to automation by ______

A

Skeggs and Hochstrasser

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8
Q

Give the process of colorimetric procedure in Cl determination

A

Chloride reacts directly with mercuric thiocyanate
= thiocyanate ions

thiocyanate ions combine with ferric ions
= reddish ferric thiocyanate

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9
Q

first reaction in the procedure

A

Chloride reacts directly with mercuric thiocyanate
= thiocyanate ions

Hg+ 2 (SCN) + 2 CL —-> HGML2 + 2(SCN)

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10
Q

2ND REACTION IN THE COLORIMETRI CPROCEDURE

A

thiocyanate ions combine with ferric ions
= reddish ferric thiocyanate

Fe+3 + 3(SCN) ——> Fe(SCN)3

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11
Q

the colorimetric method is used in what type of samples

A

serum, plasma, urine, spinal fluid

not in SWEAT

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12
Q

reagent composition of cl det

A

mercuric thiocyanate 1 mmol/l
ferric nitrate 37.5 mmol/l
mercuric nitrate
methanol and water 0.155 mol/l
nitric acid and surfactants

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13
Q

standard component

A

aqueous solution of sodium chloride

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14
Q

stability of reagent and standard

A

15-30*C until expiration labels

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15
Q

nm of cl det

A

500 nm (480-510nm)

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16
Q

blood should be drawn with a minimum venous stasis and the serum and plasma separated from cells as soon as possible to prevent diffusion of chloride into red cells

true or false

A

true

17
Q

stability of chloride in serum, plasma, urine, and spinal fluid is stable at

A

room temp and refrigerator for 1 week

18
Q

Urine is treated with what type of water

A

deionized water (1 part urine and 2 parts water) 1:3

19
Q

is bromide an interference for Chloride det?

A

yes, if bromide is present, it will be calculated as chloride

20
Q

other ions that will be calculated as chloride aside from bromide

A

iodide, thiocyanate, and sulfhydryl ions

21
Q

what should we do if bromide is involved

A

separate determination for Br is made, values converted to meq/L and the result subtracted from the reported sum of both CL and Br obtained

22
Q

bromide is encountered in ___ and ___

A

bromide intoxication or heavy sedation

23
Q

chloride residue in glassware are can be removed by a ______

A

nitric acid wash followed by rinsing with deionized or distilled water

24
Q

he published a comprehensive list of drugs and substances which may interfere with chloride det

A

young et.al

25
Q

reagent blank

reagent -
standard -
sample -

A

reagent blank

reagent - 1 ml
standard - 0
sample - 0

26
Q

standard

reagent -
standard -
sample -

A

standard

reagent - 1 ml
standard - 0.01 ml
sample - 0

27
Q

unknown/sample

reagent -
standard -
sample -

A

unknown/sample

reagent - 1 ml
standard - 0
sample - 0.01 ml

28
Q

after preparation of solution, wnat’s next?

A

incubate at room temp (15-30*C) for 5 mns

29
Q

read absorbance of U and S at what 500 nm within how many minutes ?

A

30 mns

30
Q

does the reaction follows Beer’s Law?

A

no, but ha a linear range of 70-120 mEq/L

31
Q

formula for serum Cl determination (mEq/l)

A

AU/AS * 100

32
Q

formula for urine 24 hr chloride determination (mEq/24h)

A

(mEq/L)/1000 * 24h volume (ml)

33
Q

purpose of 1000 in the formula for urine 24 hr

A

1000 converts meq/L to meq/ml

34
Q

serum or plasma ref range

A

98-106 mEq/L

35
Q

csf ref range

A

118-132 mEq/L

36
Q

urine ref range

A

110-250 mEq/24hr

37
Q

do urine’s concentration varies with diet?

A

yes

38
Q

linearity of CL determination

A

70-120 mEq/L

39
Q
A

CCHM 2 (22 decks)

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