LAB 6 - chloride Flashcards
classical methods for chloride determination have been based on the combination of this ion with either __ or ____
ion + silver
ion + mercury
classical methods for chloride determination have been based on the combination of this ion with either silver or mercury to form ___
undissociated chloride compound
most popular for the estimation of chloride in body fluids since 1941 gas has been the technique ____
titrametric technique of shales and shales
titrametric technique of shales and shales uses a standardized solution of __
mercuric nitrate and diphenylcarbazone
purpose of diphenylcarbazone in the titrametric technique of shales and shales
indicator
the colorimetric procedure (which we did in lab) is based on the water analysis scheme of ___
Zall et.al
the colorimetric procedure (which we did in lab) is based on the water analysis scheme of Zall et.al which was later adapted to automation by ______
Skeggs and Hochstrasser
Give the process of colorimetric procedure in Cl determination
Chloride reacts directly with mercuric thiocyanate
= thiocyanate ions
thiocyanate ions combine with ferric ions
= reddish ferric thiocyanate
first reaction in the procedure
Chloride reacts directly with mercuric thiocyanate
= thiocyanate ions
Hg+ 2 (SCN) + 2 CL —-> HGML2 + 2(SCN)
2ND REACTION IN THE COLORIMETRI CPROCEDURE
thiocyanate ions combine with ferric ions
= reddish ferric thiocyanate
Fe+3 + 3(SCN) ——> Fe(SCN)3
the colorimetric method is used in what type of samples
serum, plasma, urine, spinal fluid
not in SWEAT
reagent composition of cl det
mercuric thiocyanate 1 mmol/l
ferric nitrate 37.5 mmol/l
mercuric nitrate
methanol and water 0.155 mol/l
nitric acid and surfactants
standard component
aqueous solution of sodium chloride
stability of reagent and standard
15-30*C until expiration labels
nm of cl det
500 nm (480-510nm)
blood should be drawn with a minimum venous stasis and the serum and plasma separated from cells as soon as possible to prevent diffusion of chloride into red cells
true or false
true
stability of chloride in serum, plasma, urine, and spinal fluid is stable at
room temp and refrigerator for 1 week
Urine is treated with what type of water
deionized water (1 part urine and 2 parts water) 1:3
is bromide an interference for Chloride det?
yes, if bromide is present, it will be calculated as chloride
other ions that will be calculated as chloride aside from bromide
iodide, thiocyanate, and sulfhydryl ions
what should we do if bromide is involved
separate determination for Br is made, values converted to meq/L and the result subtracted from the reported sum of both CL and Br obtained
bromide is encountered in ___ and ___
bromide intoxication or heavy sedation
chloride residue in glassware are can be removed by a ______
nitric acid wash followed by rinsing with deionized or distilled water
he published a comprehensive list of drugs and substances which may interfere with chloride det
young et.al
reagent blank
reagent -
standard -
sample -
reagent blank
reagent - 1 ml
standard - 0
sample - 0
standard
reagent -
standard -
sample -
standard
reagent - 1 ml
standard - 0.01 ml
sample - 0
unknown/sample
reagent -
standard -
sample -
unknown/sample
reagent - 1 ml
standard - 0
sample - 0.01 ml
after preparation of solution, wnat’s next?
incubate at room temp (15-30*C) for 5 mns
read absorbance of U and S at what 500 nm within how many minutes ?
30 mns
does the reaction follows Beer’s Law?
no, but ha a linear range of 70-120 mEq/L
formula for serum Cl determination (mEq/l)
AU/AS * 100
formula for urine 24 hr chloride determination (mEq/24h)
(mEq/L)/1000 * 24h volume (ml)
purpose of 1000 in the formula for urine 24 hr
1000 converts meq/L to meq/ml
serum or plasma ref range
98-106 mEq/L
csf ref range
118-132 mEq/L
urine ref range
110-250 mEq/24hr
do urine’s concentration varies with diet?
yes
linearity of CL determination
70-120 mEq/L
