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CCHM 2 > LAB 5 - MAGNESIUM > Flashcards

LAB 5 - MAGNESIUM Flashcards

1
Q

the normal human body contains about ____ of magnesium

A

20-80 g

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2
Q

magnesium on our body is most stored in
what organs

A

bone and muscles

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3
Q

what will happen to organs if there’s deficiency in Mg

A

can be subjected to immobilization

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4
Q

magnesium is an essential component in many enzyme reactions either as a ___ or ____

A

metalloenzyme or an intermediary activator

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5
Q

It is the most important in those reactions involving PT and PS

what do they stand for?

A

phosphate transfer and protein synthesis

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6
Q

severe magnesium deficiency can result in impairment of __ function

A

neuromuscular

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7
Q

Symptoms usually do not occur until magnesium levels fall below _____ mEq/L

A

1 mEq/L ( mmol/l)

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8
Q

since renal clearance plays a large role in magnesium homeostasis, the _____ excretion of magnesium is also a useful index of magnesium deficiency or excess

A

urinary

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9
Q

On the lab test performed during our laboratory, what are the reagent we combine?

A

magnesium and xylidyl blue-1 under ALKALINE CONDITION

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10
Q

combining magnesium and xylidyl blue will give us water soluble _______

A

red-purple chelate

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11
Q

we perform the activity at what wavelength

A

520 mn

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12
Q

Interference from calcium is prevented by the presence of ____ which completely masks a similar colored calcium chelate

A

EGTA

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13
Q

the magnesium chelate color is STABLE, and the assay is not subject to interferences from other inorganic ions

true or false

A

true

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14
Q

magnesium reagent components

A

ethanolamine 750 mmol/l
EGTA 60 mmol/l
thiomalic acid 6.7 mmol/l
xylidyl blye 110 mmol/l

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15
Q

magnesium standard components

A

Aqueous solution of magnesium chloride with sodium aside as preservatives

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16
Q

Both reagent and standard are stable at _____*C until the expiration date on the labels

A

2-8*C

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17
Q

nm of MG determination we use in the lab

A

520 nm

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18
Q

what type of specimen is the preferred specimen

A

serum

19
Q

can we use plasma here?

A

yes but avoid EDTA as anticoagulant

20
Q

why do we need to avoid EDTA?

A

gives us lower test results

21
Q

what type of urine smple is needed for Mg determination?

A

24 hr urine

22
Q

24 hr urine must be preserved with ____

A

hydrochloric acid

23
Q

24 hr urine’s pH must be adjusted to

A

3-4

24
Q

24 hr urine must be diluted __ before analysis

A

1 part urine with 4 parts distilled water before analysis

25
Q

stability of serum and plasma in 2-8*C

A

7 days

26
Q

stability of serum and plasma in -20*C

A

1 month

27
Q

stability of treated urine in 2-8*C

A

7 days as well same as plasma

28
Q

interferences for Mg determination

A

use of EDTA
hemolysis
bilirubin up to 20 mg/dl
lipemic serum
contaminated glassware

29
Q

why hemolysis is must be avoided

A

rbc contain higher levels of magnesium

30
Q

is the greatest source of error

A

contaminated glassware

31
Q

since contaminated is the greatest source of error in Mg Determination

it is recommended to employ ___ in the procedure

A

use disposable tubes and pipette tips

32
Q

reagent blank

reagent -
standard-
sample

A

reagent blank

reagent - 1 ml
standard- 0
sample - 0

33
Q

standard blank

reagent -
standard-
sample -

A

standard blank

reagent - 1 ml
standard- 0.01 ml
sample - 0

34
Q

sample

reagent -
standard-
sample -

A

reagent - 1 ml
standard- 0
sample - 0.01 ml

35
Q

what should we do if the instrument requires volumes greater than 1 ml

A

volumes may be doubled

36
Q

step after preparing solutions needed

A

incubate the cuvettes at 37*C for 5 minutes

37
Q

after incubating at 37*C we must run the test at 520 nm within _______

A

1 hr

38
Q

formula for plasma or serum (mEq/L)

A

(Au / As) * 2

39
Q

formula for urine magnesium (mEq/L)

A

(Au / As) * 2*5

(Au / As) * 10

40
Q

samples having magnesium values greater than ____ are diluted 1:2 (1+1) with distilled water, the assay repeated and results multiplied factor of 2

A

4.3 mEq/L

41
Q

serum or plasma ref range

A

1.3-2.1 mEq/L

42
Q

24 hr urine ref range

A

6 - 10 - mEq/L

43
Q
A

CCHM 2 (22 decks)

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