LAB 9 - HCG Flashcards
compound Produced by the trophoblast cells of the placenta during pregnancy
HUMAN CHORIONIC GONADOTROPIN
human chorionic gonadotropin is a dimeric molecule which consists of
alpha sub unit and beta sub unit
is the HUMAN CHORIONIC GONADOTROPIN a glycoprotein?
yes po
Same Alpha Sub-unit is analogous to
LH, FSH, TSH
Same beta Sub-unit is analogous to
LH
Normal concentrations of LH, FSH, and TSH would not actually have significant effect on the qualitative test for HCG
or pregnancy testing.
t or f
true - it ha sthreshold
HCG
Serves to maintain ___ production by the corpus luteum in the early pregnancy
progesterone
___ is the predominant form during pregnancy
Intact HCG
HCG is can be detected after how many days of ovulation
2-3 days
For serum assay, an antibody is directed separately to _____sub-unit and intact HCG
beta
the one being detected in serum assay is the
beta sub unit and the intact hcg
For the urine assay, what is specifically measured is the ___
alpha and beta subunit of HCG.
Qualitative test for urine samples has detection limits of about __
50 mIU/mL
the test kits we use can have a sensitivity as low as ___ mIU/mL
25 - meaning it’s easier to trigger or detect a positive reaction even at a smaller concentration
Serum HCG increases above reference interval (4 – 6 mIU/mL) by __
implantation
Indicative of pregnancy or trophoblastic disease
> 5 mIU/mL
Serum level during the first trimester of pregnancy
> 100,000 mIU/mL
HCG levels increase to more than 100 mIU/mL. It can go as high as that. Hence, there is a
significant color change and the appearance of a line on our test kit.
what could be the indication
pregnancy at first trimester
antibody-antigen-antibody setup to detect the presence of a
particular antigen.
sandwich assay - immunometric method
The presence of an antigen between the two antibodies will
trigger or create the colorimetric reaction.
immunochromatographic
method using a sandwich technique
SD HCG One Step Pregnancy Test
what is its sensitivity
25 mIU/ml
As early as the first day of a missed period, it can readily provide a positive result for individuals who are pregnant. On
the first day of a missed period, it is estimated that the HCG level is around 100 mIU/mL.
SD HCG One Step Pregnancy Test
amount of HCG in 10-12 weeks into pregnancy
30,000-100,000 mIU/L
in the Test Kit
what is the component of the gold conjugate
Mouse Anti-beta hCG monoclonal antibody-gold colloid
in the Test Kit
what is the component of the test line
goat anti-hcg
in the Test Kit
what is the component of the control line
goat anti mouse IgG
specimen for HCG
first morning urine specimen
Urine samples containing visible precipitate should be __
centrifuges or allowed to settle
if the sample is stored in refrigerator, it can be kept for __ hrs
72 hrs
if the sample is stored in freezer, it can be kept for ___
14 days
in using the test kit, we squeeze how many drops of urine in the well
3-4 drops
we interpret the results at how many mns
3 mns
Negative Result –>
the presence of only one purple band (“C”) within the test window
Positive Result ->
® the presence of two color bands (“I”) and (“C”) within the result window
Invalid Results –>
® if the purple color band (“C”) is not visible within the result window
threshold for LH as an interference
200 mIU/ml
threshold for FSH as an interference
1000 mIU/ml
threshold for TSH as an interference
1000 uIU/ml
SUBSTANCES (20 mg/dL)
list the interferences in this threshold
- Acetaminophen
- Acetylsalicylic Acid
- Ampicillin
- Ascorbic Acid
- Atropine
- Caffeine
- Gentisic Acid
- Phenylpropanolamine
- Salicylic Acid
- tetreacyline
glucose has an interference of
2000 mg/dl
hemoglobin has an interference of
25 mg/dl
albumin has an interference of
2000 mg/dl
