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CCHM 2 > LAB 9 - HCG > Flashcards

LAB 9 - HCG Flashcards

1
Q

compound Produced by the trophoblast cells of the placenta during pregnancy

A

HUMAN CHORIONIC GONADOTROPIN

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2
Q

human chorionic gonadotropin is a dimeric molecule which consists of

A

alpha sub unit and beta sub unit

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3
Q

is the HUMAN CHORIONIC GONADOTROPIN a glycoprotein?

A

yes po

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4
Q

Same Alpha Sub-unit is analogous to

A

LH, FSH, TSH

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5
Q

Same beta Sub-unit is analogous to

A

LH

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6
Q

Normal concentrations of LH, FSH, and TSH would not actually have significant effect on the qualitative test for HCG
or pregnancy testing.

t or f

A

true - it ha sthreshold

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7
Q

HCG

Serves to maintain ___ production by the corpus luteum in the early pregnancy

A

progesterone

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8
Q

___ is the predominant form during pregnancy

A

Intact HCG

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9
Q

HCG is can be detected after how many days of ovulation

A

2-3 days

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10
Q

For serum assay, an antibody is directed separately to _____sub-unit and intact HCG

A

beta

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11
Q

the one being detected in serum assay is the

A

beta sub unit and the intact hcg

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12
Q

For the urine assay, what is specifically measured is the ___

A

alpha and beta subunit of HCG.

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13
Q

Qualitative test for urine samples has detection limits of about __

A

50 mIU/mL

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14
Q

the test kits we use can have a sensitivity as low as ___ mIU/mL

A

25 - meaning it’s easier to trigger or detect a positive reaction even at a smaller concentration

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15
Q

Serum HCG increases above reference interval (4 – 6 mIU/mL) by __

A

implantation

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16
Q

Indicative of pregnancy or trophoblastic disease

A

> 5 mIU/mL

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17
Q

Serum level during the first trimester of pregnancy

A

> 100,000 mIU/mL

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18
Q

HCG levels increase to more than 100 mIU/mL. It can go as high as that. Hence, there is a
significant color change and the appearance of a line on our test kit.

what could be the indication

A

pregnancy at first trimester

19
Q

antibody-antigen-antibody setup to detect the presence of a
particular antigen.

A

sandwich assay - immunometric method

20
Q

The presence of an antigen between the two antibodies will
trigger or create the colorimetric reaction.

A

immunochromatographic
method using a sandwich technique

21
Q

SD HCG One Step Pregnancy Test

what is its sensitivity

A

25 mIU/ml

22
Q

As early as the first day of a missed period, it can readily provide a positive result for individuals who are pregnant. On
the first day of a missed period, it is estimated that the HCG level is around 100 mIU/mL.

A

SD HCG One Step Pregnancy Test

23
Q

amount of HCG in 10-12 weeks into pregnancy

A

30,000-100,000 mIU/L

24
Q

in the Test Kit

what is the component of the gold conjugate

A

Mouse Anti-beta hCG monoclonal antibody-gold colloid

25
Q

in the Test Kit

what is the component of the test line

A

goat anti-hcg

26
Q

in the Test Kit

what is the component of the control line

A

goat anti mouse IgG

27
Q

specimen for HCG

A

first morning urine specimen

28
Q

Urine samples containing visible precipitate should be __

A

centrifuges or allowed to settle

29
Q

if the sample is stored in refrigerator, it can be kept for __ hrs

A

72 hrs

30
Q

if the sample is stored in freezer, it can be kept for ___

A

14 days

31
Q

in using the test kit, we squeeze how many drops of urine in the well

A

3-4 drops

32
Q

we interpret the results at how many mns

A

3 mns

33
Q

Negative Result –>

A

the presence of only one purple band (“C”) within the test window

34
Q

Positive Result ->

A

® the presence of two color bands (“I”) and (“C”) within the result window

35
Q

Invalid Results –>

A

® if the purple color band (“C”) is not visible within the result window

36
Q

threshold for LH as an interference

A

200 mIU/ml

37
Q

threshold for FSH as an interference

A

1000 mIU/ml

38
Q

threshold for TSH as an interference

A

1000 uIU/ml

39
Q

SUBSTANCES (20 mg/dL)

list the interferences in this threshold

A
  • Acetaminophen
  • Acetylsalicylic Acid
  • Ampicillin
  • Ascorbic Acid
  • Atropine
  • Caffeine
  • Gentisic Acid
  • Phenylpropanolamine
  • Salicylic Acid
  • tetreacyline
40
Q

glucose has an interference of

A

2000 mg/dl

41
Q

hemoglobin has an interference of

A

25 mg/dl

42
Q

albumin has an interference of

A

2000 mg/dl

43
Q
A

CCHM 2 (22 decks)

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