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Microbiology > lab pt1 > Flashcards

lab pt1 Flashcards

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1
Q

ns: is the media selective/differential? why is it helpful?

A

bap (differential; lysing of red blood cells)
msa (selective and differential; helps isolate S. aur)

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2
Q

what microorganism do nasal swabs screen for

A

staphylococcus aureus

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3
Q

ns: what media is set up and why

A

blood agar plate and mannitol salt agar plate

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4
Q

ns: S. aur on media

A

growth: beta hemolytic
gram stain: gram positive cocci
catalase: positive
slide coagulase: positive

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5
Q

ns: if S. aur what is the next thing to find? MRSA or MSSA isolate?

A

Screen for MRSA (methicillin resistant staphylococcus aureus)

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6
Q

ns: how do you determine if its MRSA or MSSA

A

you determine if it is MRSA with a cefoxitin disc

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7
Q

ns: how and when do you set up/read test

A

transfer 18-24 hr old culture in saline solution and plate on MH plate (streak 3 way) place cefoxtinin disc in middle
read after 18-24 hrs

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8
Q

ns: how is test resulted

A

The test will either be methicillin resistant or sensitive

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9
Q

ns: expected result for MRSA and MSSA

A

MSSA: sensitive (greater than 20mm zone)
MRSA: resistant (less than 19mm zone)

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10
Q

ns: clinical implication of patient with this test result

A
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11
Q

t: symptoms presented by patient? what is important when collecting specimen

A
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12
Q

t: pathogen you are looking for in sample

A

streptococcus pyogenes

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13
Q

t: how do you incubate these plates (AIR or CO2), what temp?

A

incubated at 35 degress celcius in CO2 incubator

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14
Q

t: what growth media are you looking for

A

colony: small beta hemolytic colonies
gram stain: gram positive cocci
catalase: negative

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15
Q

t: what test is performed to determine whether you isolated the pathogen

A

bacitracin susceptibility test

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16
Q

t: how do you set up test and what are expected results

A

inoculate BAP plate and plate bacitracin disc between the first and second quadrants; if there is a zone of inhibition than it is sensitive and positive for group A streptococci

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17
Q

t: what is patients diagnosis

A

positive for streptococcus species

18
Q

s: what diagnosis is patient being evaluated for

A
19
Q

s: under what conditions did you incubate plate

A

35 degrees celsius in CO2 incubator

20
Q

s: what is growing on media

A

colony: alpha hemolytic
gram stain: gram positive cocci
catalase: negative

21
Q

s: what is the next test performed and how do you set it up

A

optochin sensitivity test
plate isolated colones on BAP; streak first quadrant three ways and continue to streak normally then place optochin disk on the primary streak

22
Q

s: how is the test resulted and what is the expected result

A

the test is resulted as either sensitive (greater than 14mm) or negative (less than 13 mm)
the expected result is sensitive streptococcus pneumoniae isolated

23
Q

u: what are the three common pathogens

A

e coli (most common)
pseudomonas aeruginosa
klebsiella pneumoniae

24
Q

u: what will the gram stains of all three organisms be

A

gram negative rods

25
Q

u: what will all three look like on plates

A

e coli (b-hemo; lactose fermenter)
k. pne (non-hemo; mucoid lactose fermenter)
p.aer (b-hemo; nonfermenter

26
Q

u: why is urine plated to a BA and MAC )

A

BAP: records colony size, shape, and hemolysis
MAC: records whether its a lactose fermenter

27
Q

u: how was the urine plated (qualitative vs quantitative streak)

A

quantitative streak

28
Q

u: is BA/MAC selective/differential

A

BAP: differential
MAC: both selective/differential

29
Q

u: what spot biochems do you want to set up on your isolate

A
  1. oxidase
  2. spot indole
30
Q

u: what will positive/negative reactions look like

A

oxidase: positive (violet/purple)
spot: indole: positive (blue-green)

31
Q

u: what is the suspected organism you have

A

escherichia col

32
Q

rapid tests function

A

designed to perform bedside, check its not expired/stored properly, they have internal controls

33
Q

beta hemolytic

A

complete clearing (halo/zone around isolate)

34
Q

alpha hemolytic

A

partial lysis (greenish brown(

35
Q

non hemolytic

A

no lysis

36
Q

lactose fermenter on MAC

A

pink/purple

37
Q

non lactose fermenter on MAC

A

no color change on agar

38
Q

mannitol fermenter on MSA

A

yellow

39
Q

non mannitol fermenter on MSA

A

non color change

40
Q

what does positive catalase test look like

A

gas bubbles

41
Q

what does positive oxidase test look like

A

turns purple

42
Q

what does positive indole test look like

A

turns blue green

Microbiology (29 decks)

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