lab

Question 1

scopes are

Answer 1

parafocal

Question 2

final magnification

Answer 2

magnification times 10

Question 3

chemicals used in gram stain

Answer 3

crystal violet
iodine
alcohol
safranin

Question 4

functions of crystal violet, iodine, alcohol, safranin

Answer 4

crystal violet: adheres/stains cell membrane of both gram +/-
iodine: fixes crystal violet into cell wall
alcohol: decolorizes if gram -
safranin: counter strains to stain gram -

Question 5

how is organism fixed to the slide

Answer 5

heat fixation

Question 6

gram positive

Answer 6

purple; can be cocci/rods

Question 7

gram positive organisms

Answer 7

staphylococcus aureus, staphylococcus epidermis, streptococcus pneumoniae

Question 8

gram negative

Answer 8

pink; can be cocci/rods/diplococci (usually rods)

Question 9

gram negative organisms

Answer 9

escherichia coli, proteus mirabilis, pseudomonas aeruginosa

Question 10

most common agar that most specimens and isolates are plated to

Answer 10

Blood agar plate

Question 11

why is this agar most commonly used

Answer 11

sheep blood provides enrichment to support the growth of microorganisms and aids in identification based on hemolytic activity

Question 12

beta hemolysis

Answer 12

complete clearing

Question 13

beta hemolysis example

Answer 13

streptococcus pyogenes

Question 14

alpha hemolysis

Answer 14

partial clearing/greening

Question 15

alpha hemolysis example

Answer 15

streptococcus pneumoniae

Question 16

nonhemolytic

Answer 16

no lysis/clearing

Question 17

nonhemolytic example

Answer 17

group B streptococci