lab final

Question 1

nasal culture

Answer 1

looking for staph aur.
plate to bap and msa
–> if hemolytic: gram stain, catalase, slide coagulase, cefoxitin disc test

Question 2

final reporting nasal

Answer 2

no growth = no growth
growth but no fermentation on msa = culture negative for staphylococcus aureus
staph present= all tests positive and msa fermentation
not mrsa: methicillin sensitive staphylococcus aureus
mrsa: methicillin resistant staphylococcus aureus

Question 3

throat culture

Answer 3

looking for streptocuccus
plate on bap
–> if beta hemolytic: gram stain, catalase, bacitracin

Question 4

final reporting throat

Answer 4

no beta hemolytic: culture negative for streptococcus species group
gram +, catalase -, bacitracin sensitive: streptococcus species group a isolated

Question 5

sputum culture

Answer 5

either streptococcus pneumoniae isolated or viridans streptococcus isolated
alpha hemolytic colonies
–> run gram stain, catalase, optochin

Question 6

final reporting sputum

Answer 6

strep. pneumoniae: gram+, catalase -, optochin sensitive
viridans strep: gram+, catalase - , optochin resistant

Question 7

urine tests

Answer 7

bap, mac, gram, spot oxidase, indole

Question 8

urine final reporting

Answer 8

e. coli: bap (beta), mac (lf), gram (gnr), oxidase (-) indole (+)
k. pne: bap (non), mac (mlf), gram (gnr), oxidase (-) indole (-)
p. aer: bap (beta), mac (nf), gram (gnr), oxidase (+) indole (-)

Question 9

stool tests

Answer 9

gram, mac, hea, oxidase, indole, mr, vp, tsi, urea, citrate

Question 10

stool final reporting

Answer 10

mac: ecoli (lf), k. aer (lf), shigella (nlf), salmonella (nlf)
hea: ecoli (ferm/no), k.aer (ferm), shigella (non ferm), salmonella (non ferm w h2s+)
oxidase: all neg
tsi: ecoli (a/a), kaer (a/a or k/a), shigella (k/a), salmonella (k/a w h2s+)
urea: all neg
mr: only k.aer neg
vp: only k. aer pos
indole: only e. coli pos
citrate: k. aer and salmonella pos