Lab 1 Flashcards
What biosafety level has low risk microbes that pose little to no threat of infection in healthy adults
BSL-1
Non-pathogenic E. Coli can be in what BSL
BSL-1
What is BSL-2 characterized by
Working with agents associated with human diseases that pose a moderate health hazard
What BSL is equine encephalitis, HIV, S. Aureus, P. Multicoda
BSL-2
What are some safety measures taken in BSL-2 labs
Risk of aerosol or splashes must be done in biosafety cabinet, autoclave, self closing/locking doors with biohazard signs, sink and eyewash stations
BSL-2 Ag are veterinary specific what organisms could be present here
Scabies, chronic wasting disease, prions
What is BSL-3 characterized by
Infectious agents that may be aerosolized and cause serious or potentially lethal diseases
What are some examples of BSL-3 organisms
M. Tuberculosis, Bartonella henselae, West Nile virus
What are some additional restrictions/ safety measures in BSL-3
Restricted access, vaccinations, respirators, controlled airflow
What characterizes BSL-4
Highly deadly and infectious agents
What are some safety measures taken in BSL-4
Clothing specific to lab that can’t be worn outside facility, autoclaved clothing, showering in and out, pass through air locked chamber, ventilation suits
What is the amines bacterial transport medium with charcoal good for? How does the charcoal work
Transport aerobic and anaerobic bacteria such as fungal agents. Charcoal binds agent for products of metabolism and may inhibit or destroy some sensitive bacteria
What is the amines bacterial transport medium charcoal not good for
Anaerobic organisms
What transport medium is good for suspected mycoplasma
Amines bacterial transport without charcoal
What is anaerobic bacterial transport medium good for
Transporting anaerobic organisms in GI tract, abscesses
Color changing dye to show oxygen exposure