Lab 1 Flashcards

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1
Q

What biosafety level has low risk microbes that pose little to no threat of infection in healthy adults

A

BSL-1

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1
Q

Non-pathogenic E. Coli can be in what BSL

A

BSL-1

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2
Q

What is BSL-2 characterized by

A

Working with agents associated with human diseases that pose a moderate health hazard

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3
Q

What BSL is equine encephalitis, HIV, S. Aureus, P. Multicoda

A

BSL-2

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4
Q

What are some safety measures taken in BSL-2 labs

A

Risk of aerosol or splashes must be done in biosafety cabinet, autoclave, self closing/locking doors with biohazard signs, sink and eyewash stations

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5
Q

BSL-2 Ag are veterinary specific what organisms could be present here

A

Scabies, chronic wasting disease, prions

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6
Q

What is BSL-3 characterized by

A

Infectious agents that may be aerosolized and cause serious or potentially lethal diseases

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7
Q

What are some examples of BSL-3 organisms

A

M. Tuberculosis, Bartonella henselae, West Nile virus

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8
Q

What are some additional restrictions/ safety measures in BSL-3

A

Restricted access, vaccinations, respirators, controlled airflow

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9
Q

What characterizes BSL-4

A

Highly deadly and infectious agents

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10
Q

What are some safety measures taken in BSL-4

A

Clothing specific to lab that can’t be worn outside facility, autoclaved clothing, showering in and out, pass through air locked chamber, ventilation suits

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11
Q

What is the amines bacterial transport medium with charcoal good for? How does the charcoal work

A

Transport aerobic and anaerobic bacteria such as fungal agents. Charcoal binds agent for products of metabolism and may inhibit or destroy some sensitive bacteria

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12
Q

What is the amines bacterial transport medium charcoal not good for

A

Anaerobic organisms

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13
Q

What transport medium is good for suspected mycoplasma

A

Amines bacterial transport without charcoal

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14
Q

What is anaerobic bacterial transport medium good for

A

Transporting anaerobic organisms in GI tract, abscesses

Color changing dye to show oxygen exposure

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15
Q

What are the blood culture bottles good for

A

Aerobic or anaerobic organisms from blood, CSF or urine collected sterilely

Small samples so bacteria can grow

16
Q

What is the Stuart transport medium good for

A

Transporting aerobic +/- anaerobic organisms but anaerobic organisms can be affected

17
Q

What is the Cary Blair transport medium good for

A

Modified Stuart’s with improved buffers and organic composition so can transfer anaerobic and aerobic

Useful for salmonella and shigella

18
Q

What are sterile swabs without media good for

A

If PCR is diagnostic test and no culture

19
Q

What is TSA 5% sheep blood agar good for

A

Simple aerobic or anaerobic growth of any bacterial agent, allows for differentiating hemolysis

20
Q

What is MacConkeys agar used for

A

Gram negative, purple hue if bacteria fermenting lactose

21
Q

What is mannitol salt sugar agar used for

A

Gram positive

22
Q

What is alpha hemolysis

A

Partial greenish halo due to hydrogen peroxide produced y bacteria acting on hemoglobin to produce methemoglobin

23
Q

What is beta hemolysis

A

Complete hemolysis result of enzyme streptolysin produced by specific bacteria like S. Aureus

24
Q

What is gamma hemolysis

A

Absence of hemolysis

25
Q

what is light (bright field, compound) microscope good for

A

viewing living or stained bacteria