Lab 1 Flashcards
What biosafety level has low risk microbes that pose little to no threat of infection in healthy adults
BSL-1
Non-pathogenic E. Coli can be in what BSL
BSL-1
What is BSL-2 characterized by
Working with agents associated with human diseases that pose a moderate health hazard
What BSL is equine encephalitis, HIV, S. Aureus, P. Multicoda
BSL-2
What are some safety measures taken in BSL-2 labs
Risk of aerosol or splashes must be done in biosafety cabinet, autoclave, self closing/locking doors with biohazard signs, sink and eyewash stations
BSL-2 Ag are veterinary specific what organisms could be present here
Scabies, chronic wasting disease, prions
What is BSL-3 characterized by
Infectious agents that may be aerosolized and cause serious or potentially lethal diseases
What are some examples of BSL-3 organisms
M. Tuberculosis, Bartonella henselae, West Nile virus
What are some additional restrictions/ safety measures in BSL-3
Restricted access, vaccinations, respirators, controlled airflow
What characterizes BSL-4
Highly deadly and infectious agents
What are some safety measures taken in BSL-4
Clothing specific to lab that can’t be worn outside facility, autoclaved clothing, showering in and out, pass through air locked chamber, ventilation suits
What is the amines bacterial transport medium with charcoal good for? How does the charcoal work
Transport aerobic and anaerobic bacteria such as fungal agents. Charcoal binds agent for products of metabolism and may inhibit or destroy some sensitive bacteria
What is the amines bacterial transport medium charcoal not good for
Anaerobic organisms
What transport medium is good for suspected mycoplasma
Amines bacterial transport without charcoal
What is anaerobic bacterial transport medium good for
Transporting anaerobic organisms in GI tract, abscesses
Color changing dye to show oxygen exposure
What are the blood culture bottles good for
Aerobic or anaerobic organisms from blood, CSF or urine collected sterilely
Small samples so bacteria can grow
What is the Stuart transport medium good for
Transporting aerobic +/- anaerobic organisms but anaerobic organisms can be affected
What is the Cary Blair transport medium good for
Modified Stuart’s with improved buffers and organic composition so can transfer anaerobic and aerobic
Useful for salmonella and shigella
What are sterile swabs without media good for
If PCR is diagnostic test and no culture
What is TSA 5% sheep blood agar good for
Simple aerobic or anaerobic growth of any bacterial agent, allows for differentiating hemolysis
What is MacConkeys agar used for
Gram negative, purple hue if bacteria fermenting lactose
What is mannitol salt sugar agar used for
Gram positive
What is alpha hemolysis
Partial greenish halo due to hydrogen peroxide produced y bacteria acting on hemoglobin to produce methemoglobin
What is beta hemolysis
Complete hemolysis result of enzyme streptolysin produced by specific bacteria like S. Aureus
What is gamma hemolysis
Absence of hemolysis
what is light (bright field, compound) microscope good for
viewing living or stained bacteria
