I: Overview and Enteric Lab Flashcards
Gram Positive or Gram Negative? Endotoxin/LPS
Negative
Gram Positive or Gram Negative? Outer membrane
Negative
Gram Positive or Gram Negative? Teichoic Acids
Positive
Gram Positive or Gram Negative? Thick Cell Wall
Positive
Name four pathogens that are major contaminants of meat products.
Campylobacter jejuni, Salmonella, E. coli, Listeria monocytogenes
Name the 4 Gram staining steps for a fixed bacterial smear.
Hucker’s crystal violet, Iodine, Decolorize, Safranin
Name the toxin(s) of the pathogen: EHEC
Shigella-like toxin
Name the toxin(s) of the pathogen: ETEC
Heat-stable enterotoxin, Cholera-like toxin
Name two pathogens associated with dysentery.
Entamoeba histolytica, Shigella dysenteriae
Name two pathogens that may be identified by curved or spiral shape on gram stain of a stool specimen.
Campylobacter jejuni, Vibrio cholerae
On MacConkey agar, lactose(-) colonies appear ______.
Clear/Colorless
On MacConkey agar, lactose(+) colonies appear ______.
Red
Which E. coli presentation? Dysentery-like diarrhea, severe inflammation, fever
EIEC (Invasive)
Which E. coli presentation? Pediatric diarrhea, hemorrhagic colitis, intense inflammatory response, can be complicated by Hemolytic-Uremic Syndrome
EHEC
Which E. coli presentation? Persistent diarrhea in young children without inflammation or fever
EAEC (Aggregative)
Which E. coli presentation? Usually infantile diarrhea, watery diarrhea with blood, some inflammation but no fever
EPEC (Pathogenic)
Which E. coli presentation? Watery diarrhea in infants and travelers; no inflammation or fever
ETEC
Which pathogen? Gram Negative Rods, Lactose(-), Oxidase(+)
Pseudomonas
Which pathogen? Gram Negative Rods, Lactose(+), Indole(-)
Klebsiella, Enterobacter
Which pathogen? Gram Negative Rods, Lactose(+), Indole(+)
E. coli
Which pathogen? Gram Positive Cocci, Catalase(-), alpha-hemolysis
Streptococcus pneumoniae or viridans
Which pathogen? Gram Positive Cocci, Catalase(-), beta-hemolysis
Group A, B, C, G Streptococcus
Which pathogen? Gram Positive Cocci, Catalase(+), Coagulase(-)
Coagulase-Negative Staph (Epidermidis, saphrophyticus)
Which pathogen? Gram Positive Cocci, Catalase(+), Coagulase(+)
Staph. Aureus
Which pathogen? Gram-Negative Cocci
Neisseria, Moraxella
Which pathogen? Large Gram Positive Rods
Clostridium
Which pathogen? Non-staining due to lack of cell wall
Mycoplasma, Chlamydia
Which pathogen? Small Gram Positive Rods
Listeria
Which pathogens in the lab were capable of causing laboratory-acquired infection?
Salmonella typhinurium and enteritidis, and Campylobacter jejuni
