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MICROBIOLOGY > EXERCISE 4 > Flashcards

EXERCISE 4 Flashcards

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Biology 101 flashcards
1
Q

Purpose of Culture Media

A

nutrient materials for growing and cultivating microorganisms

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2
Q

basic elements required by microorganisms

A

C, H, O, N, P, S

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3
Q

Interval for each ph classification

A

acidophile <5.5
neutrophile 5.5-7.999
alkaliphile 8 and greater

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4
Q

most common gelling agent and why

A

agar = no nutritive value and very few can degrade it

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5
Q

2 types of culture media based on chemical composition

A
  1. synthetic
  2. complex (broth and extracts and digests)
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6
Q

2 types of complex culture media

A

nutrient agar and eosin methylene blue agar

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7
Q

3 types of culture media based on consistency

A

LIQUID - 0 (growing)
SEMI-SOLID - 0.5-0.7 (bacterial motility)
SOLID - 1.5-2.0% (Enumeration, isolation, and characterization)

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8
Q

examples for each culture media

A
  1. General Purpose (Nutrient Agar and Potato Dextrose Agar)
  2. Selective (Mannitol Salt Agar) staphylococcus
  3. Differential (MacConkey Agar & Eosin Methylene Blue Agar for ecoli)
  4. Enrichment (Blood Agar and Lactose Broth)
  5. Characterization (Citrate Agar)
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9
Q

Volume for petri dish

A

15-20 mL

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10
Q

Volume for BIG TEST TUBE (18 mm)

  • slants
  • stab/broths
A
  • 6 - 8 mL
  • 10 mL
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11
Q

Volume for SMALL TEST TUBES (13 mm)

  • slant
    -stab/broth
A
  • 3 mL
  • 5 mL
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12
Q

Volume for erlenmeyer flask

A

< 60%

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13
Q

Percentage for NUTRIENT AGAR PREPARATION

A

Peptone - 0.5%
Beef Extract - 0.3%
Agar: 1.5%

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14
Q

most common liquid medium used

A

nutrient broth

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15
Q

What substance to put in
1. Erlenmeyer flask
2. Beaker

A
  1. Agar
  2. Remaining substances
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16
Q

Types of pH indicators

A

Bromcresol green
yellow - 3.8
green - 4
blue - 5.4

Bromthymol blue
yellow - 6
green - 7
blue - 7.6

Phenolphthalein
colorless - 8 or less
pink - 9
red - 10

17
Q

Example of basic substances (2) and acidic substances (1)

A

Basic: KOH and NaOH
Acidic: HCL

MICROBIOLOGY (23 decks)

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