EXERCISE 2 Flashcards
A___ T___
- precautionary measure to avoid entry of c_____
- ensures p___ of the culture
Aseptic Techniques
- contaminants
- purity
Where to usually perform experiments to ensure a___ flow and s___conditions?
BIOSAFETY CABINET for AIR flow and STERILE conditions
if BIOSAFETY is not available?
experiment on an OPEN-AIR BENCH-TOP AREA
How to disinfect work area? (2)
- 70% alcohol or or quaternary
ammonium compounds - use ALCOHOL LAMP as fire minimize chance of airborne microbes
DIFFERENCE between WET MOUNT and HANGING DROP in terms of slide?
WM: Plain slide
HD: Depression slide
DIFFERENCE between WET MOUNT and HANGING DROP in terms of preparation?
WM: WATER or LACTOPHENOL (organisms from culture slant)
HD: DROP OF SAMPLE
DIFFERENCE between WET MOUNT and HANGING DROP in terms of examination?
WM: COMMON type for living organisms
HD: Prolonged examination for GROWTH of cells in a microculture
What kind of culture do samples to be used for WET MOUNTS come from?
culture slants
WET MOUNT PREPARATION
1. Broth
2. Solid Media//Surface Growth
3. Molds
- Place a drop
- Place on water then mix
- Place on LACTOPHENOL
2 examples of sample preparation for microscopic observations
wet mount
hanging drop
what kind of movement do non-motile organisms or tiny particles in suspension exhibit??
an oscillating/quivering motion
BROWNIAN MOVEMENT
after viewing a specimen, how can you clean the slide?
immerge in 70% alcohol
HANGING DROP PREPARATION
COVERSLIP
1. 4 water droplets on each corner
2. Sample in the middle
DEPRESSION SLIDE
1. Turn over and place the depression part over the sample
Name the specimens under each sample preparation
HD
1. Carmine dye
2. Protozoan Culture (hay infusion)
3. Bacterial Culture (Proteus sp., Bacillus sp., Staphylococcus sp., & Micrococcus luteus)
WM
1. Mold Culture (Aspergillus niger & Rhizopus sp.)
2. Yeast Culture (Saccharomyces cerevisae & Schizosaccharomcyes sp.)
3. Algal Culture (Chlorella)
mode of reproduction of S. cerevisae
budding
