Chapter 9 Flashcards
1
Q
A spectrophotometer measures the amount of _____ absorbed by (or transmitted through) a sample.
A
Light
2
Q
The tip of a 10mL pipette holds how much?
A
1 mL
3
Q
What type of software will generate an absorbance spectrum?
A
LoggerPro
4
Q
When generating an absorbance standard curve, ______ is on the y-axis and _____ is on the x-axis.
A
Absorbance is the y-axis and concentration is the x-axis.
5
Q
How does a spectrophotomer work?
A
Light source -> collimator (lens) -> monochromator (prism) -> wavelength selector (slit) -> incident beam -> sample solution (cuvette) -.> emergent beam -> detector -> display
6
Q
What is on the x-axis of an absorption spectrum graph?
A
Wavelength
