CHAPTER 3 PART 1 REVIEWER Flashcards by Arriane Cyrel (MTI) | Brainscape

Q

AST systematic name

A

E.C. 2.6.1.1

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

ALT systematic name

A

E.C. 2.6.1.2

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

CK systematic name

A

E.C. 2.7.3.2

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

LDH systematic name

A

E.C. 1.1.1.27

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

AST former name

A

Serum glutamic-oxalocacetic transaminase

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

ALT former name

A

Serum glutamic pyruvic transaminase

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

2 isoenzymes of AST

A

ytoplasmic
mitochondrial

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Isoenzyme of severe tissue damage

A

mitochondrial

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Cirrhosis ULN

A

4x

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

AST levels are NOT useful in the diagnosis

A

TRUE

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Products of ALT in reverse reaction

A

Alanine
Alpha-ketoglutarate

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Products of AST in forward reaction

A

Oxaloacetate
Glutamate

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

ALT is more liver specific

A

TRUE

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

AST:ALT ratio

A

De Ritis Ratio

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Karmen Method wavelength

A

340 nm

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

AST indicator enzyme

A

Malate Dehydrogenase

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

ALT indicator enzyme

A

Lactate Dehydrogenase

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

AST activity in RBC

A

15x

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

major phosphorylated compound in muscle

A

Phosphocreatine

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

CK forward reaction optimal pH

A

9

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Products of CK in reverse reaction

A

Creatine
Adenosine Triphosphate

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

LDH forward reaction pH

A

8.8-8.9

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

LDH hydrogen acceptor

A

NAD

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Oliver-Rosalki reverse reaction enzymes

A

Creatine Kinase
Hexokinase
Glucose-6-Phosphate Dehydrogenase

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Alcohol dehydrogenase

A

LDH6

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

S. muscle injury

A

LDH5

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Hepatic injury

A

LDH4

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

P. embolism, lymphocytosis and acute pancreatitis

A

LDH3

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Highest distribution of LDH subclass

A

LDH2

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Myocardial infarct and hemolytic anemia

A

LDH1

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Reference method for CK

A

Electrophoresis

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

Hypothyroidism

A

CK-MM

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

predominates in skeletal & cardiac muscle

A

CK-MM

Q

Constitutes up to 15% CK activity in the heart

A

CK- Mr

Q

can be a useful tumor marker

A

CK-BB

Q

obligate activator in CK

A

Magnesium

Q

obligate coenzyme of aminotransferase

A

Pyridoxal phosphate

Q

Products of AST in reverse reaction

A

Aspartate
Alpha-ketoglutarate

Q

Products of ALT in forward reaction

A

Pyruvate
Glutamate

Q

Products of CK in forward reaction

A

Phosphocreatine
Adenosine Diphosphate

Q

Products of LDH in forward reaction

A

Pyruvate
NADH

Q

Products of LDH in reverse reaction

A

L-Lactate
NAD

Q

Products of indicator reaction catalyzed by MDH

A

Malate
NAD

Q

Substrates of indicator reaction catalyzed by MDH

A

Oxaloacetate
NADH
H+

Q

Products of indicator reaction catalyzed by LDH

A

Lactate
NAD

Q

Substrates of indicator reaction catalyzed by LDH

A

Pyruvate
NADH
H+

Q

Oliver-Rosalki forward reaction enzymes

A

Creatine kinase
Pyruvate kinase
Lactate Dehydrogenase

Q

Products of indicator reaction catalyzed by PK

A

Pyruvate
Adenosine Triphosphate

Q

Substrates of indicator reaction catalyzed by PK

A

Adenosine Diphosphate
Phosphoenol pyruvate

Q

Substrates of indicator reaction catalyzed by LDH

A

Pyruvate
NADH
H+

Q

Products of indicator reaction catalyzed by PK

A

ADP
G6PD

Q

Substrates of indicator reaction catalyzed by PK

A

ATP
Glucose

Q

Products of indicator reaction catalyzed by G6PD

A

6-Phosphogluconate
NADPH
H

Q

Substrates of indicator reaction catalyzed by G6PD

A

Glucose-6-Phosphate
NADP

Q

Viral hepatitis ULN

A

100x

Q

Skeletal muscle disorders: Muscular dystrophies & inflammatory conditions ULN

A

4-8x

Q

AST activity in RBC

A

15x

Q

muscular dystrophy ULN

A

50x

Q

AST Karmen Method Optimal pH

A

7.3 – 7.8

Q

CK reverse reaction optimal pH

A

6.8

Q

LDH reverse reaction pH

A

7.4-7.8

Q

Wacker Method (forward rxn)

A

pH8.8

Q

Wrobleuski La Due (reverse rxn)

A

pH7.2

Q

LDH activity in RBC

A

100 - 150x

Q

AST Karmen Method Reference Range

A

5 – 30U/L(37°C)

Q

ALT Reference Range

A

6 – 37U/L(37°C)

Q

Reference range TOTAL CK in Male

A

15 – 160U/L(37°C)

Q

Reference range TOTAL CK in Female

A

15 – 130U/L(37°C)

Q

Reference range CKMB

A

< 6% Total CK

Q

LDH Reference range

A

100 – 225U/L(37°C)

Q

AST peak, rise, return to normal

A

6-8hrs, 24hrs, 5days

Q

CKMB peak, rise, return to normal

A

4-8hrs, 12-24hrs, 48-72hrs

Q

25 – 46% of CK activity

A

CKMB

Q

major CK in serum of healthy people

A

CKMM

Q

heart muscle activity

A

CKMM

Q

CK level: sensitive indicator of AMI

A

Total CK & CK-2

Q

Cardiac trauma ff. heart surgery (including transplantation)

A

Elevation of Total CK

Q

cardiac conditions

A

CKMB

Q

head injury and acute cerebrovascular disease

A

CKBB

Q

Reye’s syndrome

A

CKBB

Q

rarely seen in the serum bec. Of its molecular size

A

CKBB

Q

Extensive damage to the brain may lead to the leakage of it in the serum

A

CKBB