CHAPTER 3 PART 1 REVIEWER

Question 1

AST systematic name

Answer 1

E.C. 2.6.1.1

Question 2

ALT systematic name

Answer 2

E.C. 2.6.1.2

Question 3

CK systematic name

Answer 3

E.C. 2.7.3.2

Question 4

LDH systematic name

Answer 4

E.C. 1.1.1.27

Question 5

AST former name

Answer 5

Serum glutamic-oxalocacetic transaminase

Question 6

ALT former name

Answer 6

Serum glutamic pyruvic transaminase

Question 7

2 isoenzymes of AST

Answer 7

ytoplasmic
mitochondrial

Question 8

Isoenzyme of severe tissue damage

Answer 8

mitochondrial

Question 9

Cirrhosis ULN

Answer 9

4x

Question 10

AST levels are NOT useful in the diagnosis

Answer 10

TRUE

Question 11

Products of ALT in reverse reaction

Answer 11

Alanine
Alpha-ketoglutarate

Question 12

Products of AST in forward reaction

Answer 12

Oxaloacetate
Glutamate

Question 13

ALT is more liver specific

Answer 13

TRUE

Question 14

AST:ALT ratio

Answer 14

De Ritis Ratio

Question 15

Karmen Method wavelength

Answer 15

340 nm

Question 16

AST indicator enzyme

Answer 16

Malate Dehydrogenase

Question 17

ALT indicator enzyme

Answer 17

Lactate Dehydrogenase

Question 18

AST activity in RBC

Answer 18

15x

Question 19

major phosphorylated compound in muscle

Answer 19

Phosphocreatine

Question 20

CK forward reaction optimal pH

Answer 20

9

Question 21

Products of CK in reverse reaction

Answer 21

Creatine
Adenosine Triphosphate

Question 22

LDH forward reaction pH

Answer 22

8.8-8.9

Question 23

LDH hydrogen acceptor

Answer 23

NAD

Question 24

Oliver-Rosalki reverse reaction enzymes

Answer 24

Creatine Kinase
Hexokinase
Glucose-6-Phosphate Dehydrogenase

Question 25

Alcohol dehydrogenase

Answer 25

LDH6

Question 26

S. muscle injury

Answer 26

LDH5

Question 27

Hepatic injury

Answer 27

LDH4

Question 28

P. embolism, lymphocytosis and acute pancreatitis

Answer 28

LDH3

Question 29

Highest distribution of LDH subclass

Answer 29

LDH2

Question 30

Myocardial infarct and hemolytic anemia

Answer 30

LDH1

Question 31

Reference method for CK

Answer 31

Electrophoresis

Question 32

Hypothyroidism

Answer 32

CK-MM

Question 33

predominates in skeletal & cardiac muscle

Answer 33

CK-MM

Question 34

Constitutes up to 15% CK activity in the heart

Answer 34

CK- Mr

Question 35

can be a useful tumor marker

Answer 35

CK-BB

Question 36

obligate activator in CK

Answer 36

Magnesium

Question 37

obligate coenzyme of aminotransferase

Answer 37

Pyridoxal phosphate

Question 38

Products of AST in reverse reaction

Answer 38

Aspartate Alpha-ketoglutarate

Question 39

Products of ALT in forward reaction

Answer 39

Pyruvate Glutamate

Question 40

Products of CK in forward reaction

Answer 40

Phosphocreatine Adenosine Diphosphate

Question 41

Products of LDH in forward reaction

Answer 41

Pyruvate NADH

Question 42

Products of LDH in reverse reaction

Answer 42

L-Lactate NAD

Question 43

Products of indicator reaction catalyzed by MDH

Answer 43

Malate NAD

Question 44

Substrates of indicator reaction catalyzed by MDH

Answer 44

Oxaloacetate NADH H+

Question 45

Products of indicator reaction catalyzed by LDH

Answer 45

Lactate NAD

Question 46

Substrates of indicator reaction catalyzed by LDH

Answer 46

Pyruvate NADH H+

Question 47

Oliver-Rosalki forward reaction enzymes

Answer 47

Creatine kinase Pyruvate kinase Lactate Dehydrogenase

Question 48

Products of indicator reaction catalyzed by PK

Answer 48

Pyruvate Adenosine Triphosphate

Question 49

Substrates of indicator reaction catalyzed by PK

Answer 49

Adenosine Diphosphate Phosphoenol pyruvate

Question 50

Substrates of indicator reaction catalyzed by LDH

Answer 50

Pyruvate NADH H+

Question 51

Products of indicator reaction catalyzed by PK

Answer 51

ADP G6PD

Question 52

Substrates of indicator reaction catalyzed by PK

Answer 52

ATP Glucose

Question 53

Products of indicator reaction catalyzed by G6PD

Answer 53

6-Phosphogluconate NADPH H

Question 54

Substrates of indicator reaction catalyzed by G6PD

Answer 54

Glucose-6-Phosphate NADP

Question 55

Viral hepatitis ULN

Answer 55

100x

Question 56

Skeletal muscle disorders: Muscular dystrophies & inflammatory conditions ULN

Answer 56

4-8x

Question 57

AST activity in RBC

Answer 57

15x

Question 58

muscular dystrophy ULN

Answer 58

50x

Question 59

AST Karmen Method Optimal pH

Answer 59

7.3 – 7.8

Question 60

CK reverse reaction optimal pH

Answer 60

6.8

Question 61

LDH reverse reaction pH

Answer 61

7.4-7.8

Question 62

Wacker Method (forward rxn)

Answer 62

pH8.8

Question 63

Wrobleuski La Due (reverse rxn)

Answer 63

pH7.2

Question 64

LDH activity in RBC

Answer 64

100 - 150x

Question 65

AST Karmen Method Reference Range

Answer 65

5 – 30U/L(37°C)

Question 66

ALT Reference Range

Answer 66

6 – 37U/L(37°C)

Question 67

Reference range TOTAL CK in Male

Answer 67

15 – 160U/L(37°C)

Question 68

Reference range TOTAL CK in Female

Answer 68

15 – 130U/L(37°C)

Question 69

Reference range CKMB

Answer 69

< 6% Total CK

Question 70

LDH Reference range

Answer 70

100 – 225U/L(37°C)

Question 71

AST peak, rise, return to normal

Answer 71

6-8hrs, 24hrs, 5days

Question 72

CKMB peak, rise, return to normal

Answer 72

4-8hrs, 12-24hrs, 48-72hrs

Question 73

25 – 46% of CK activity

Answer 73

CKMB

Question 74

major CK in serum of healthy people

Answer 74

CKMM

Question 75

heart muscle activity

Answer 75

CKMM

Question 76

CK level: sensitive indicator of AMI

Answer 76

Total CK & CK-2

Question 77

Cardiac trauma ff. heart surgery (including transplantation)

Answer 77

Elevation of Total CK

Question 78

cardiac conditions

Answer 78

CKMB

Question 79

- head injury and acute cerebrovascular disease

Answer 79

CKBB

Question 80

- Reye’s syndrome

Answer 80

CKBB

Question 81

- rarely seen in the serum bec. Of its molecular size

Answer 81

CKBB

Question 82

- Extensive damage to the brain may lead to the leakage of it in the serum

Answer 82

CKBB