cc lab Flashcards
1
Q
CHOL
Patient should be seated atleast
A
5 mins prior to collection
2
Q
Fasting time for chol
A
12 - 14hrs
3
Q
tube for chol
A
gel separator
4
Q
Principle of chol estimation
A
dehy and oxi of chol to form a colored compound (colorimetry)
5
Q
2 reaction of chol est
A
Liebermann -Burchardt reac
Salkowski reac
6
Q
color of liebermann
A
green
7
Q
color of salkowski
A
red
8
Q
components of liebermann
A
(GACS)
Glacial acetic acid
Acetic Anhydride
Con sulfuric acid
9
Q
components of salkowski
A
Chol
2 sulfuric acid
ferric ion
10
Q
Precipitation of free chol
A
precipitation
11
Q
formation of colored compund
A
colorimetry
12
Q
hydrolysis of chol esters
A
saponification
13
Q
removal of protein interference
A
extraction
14
Q
reagent of saponification
A
alcoholic KOH
15
Q
Reagent of extraction
A
ethanol ether
