cc lab

Question 1

CHOL
Patient should be seated atleast

Answer 1

5 mins prior to collection

Question 2

Fasting time for chol

Answer 2

12 - 14hrs

Question 3

tube for chol

Answer 3

gel separator

Question 4

Principle of chol estimation

Answer 4

dehy and oxi of chol to form a colored compound (colorimetry)

Question 5

2 reaction of chol est

Answer 5

Liebermann -Burchardt reac
Salkowski reac

Question 6

color of liebermann

Answer 6

green

Question 7

color of salkowski

Answer 7

red

Question 8

components of liebermann

Answer 8

(GACS)
Glacial acetic acid
Acetic Anhydride
Con sulfuric acid

Question 9

components of salkowski

Answer 9

Chol
2 sulfuric acid
ferric ion

Question 10

Precipitation of free chol

Answer 10

precipitation

Question 11

formation of colored compund

Answer 11

colorimetry

Question 12

hydrolysis of chol esters

Answer 12

saponification

Question 13

removal of protein interference

Answer 13

extraction

Question 14

reagent of saponification

Answer 14

alcoholic KOH

Question 15

Reagent of extraction

Answer 15

ethanol ether

Question 16

reagent of precipitation

Answer 16

digitonin

Question 17

one step

Answer 17

color
Pearson, stern mac gavack (PSM)

Question 18

two step

Answer 18

extrac, color
petroleum ether, bloors

Question 19

three step

Answer 19

sapo, extrac, color
alcoholic KOH, abell kendall

Question 20

four step

Answer 20

prec, sapo, extrac, color
shoensheiimer, sperry, parekh and jung (SSPJ)