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3rd year > HEMA LABBB > Flashcards

HEMA LABBB Flashcards

1
Q

measures the number of red blood cells in a volume of blood

A

RBC count

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2
Q

Normal Values
Male:
Female:
Newborn:

A

4.2-5.4 x 1012/L
3.6-5.0 x 1012/L
5.5-6.5 x 1012/L

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3
Q

Helps differentiate types of anemia
(microcytic, macrocytic)

A

Anemia Evaluation

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4
Q

Identifies increased RBC mass

A

Polycythemia Screening

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5
Q

Evaluates the effectiveness of
therapies like erythropoietin

A

Monitoring treatments

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6
Q

Provides a reference method to validate automated counts.

A

Manual Counting

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7
Q

The space between the top of the platform
and cover glass is

A

0.1mm

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8
Q

Each large square is

A

1mm wide and 1 mm long

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9
Q

the entire ruled area is

A

9mm2

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10
Q

The volume of one entire platform is

A

0.9uL

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11
Q

What type of pipette is used for RBC counting

A

Thoma RBC pipet (1:200)

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12
Q

RBC diluting fluid

A

a. Trisodium citrate with formalin
b. Hayem’s solution
c.Isotonic saline, isotonic Gower’s solution and Citrate formalin solution

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13
Q

causes roleaux and agglutination in patients with hyperglobulinemia

A

Hayem’s solution

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14
Q

COMPUTATION FOR RBC COUNT

A

No. of cells counted x Correction for Dilution x Correction for Volumex106

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15
Q

Dilution

A

200

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16
Q

Correction for Volume

A

50

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17
Q

Specimen for peripheral blood smear

A

EDTA-anticoagulated whole blood
Free-flowing capillary blood

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18
Q

The most commonly used stain in the hematology laboratory

A

Romanowsky-type stain

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19
Q

is defined as any stain containing methylene blue and/or its products of oxidation and a halogenated fluorescein dye, usually eosin B or Y

A

Romanowsky stain

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20
Q

are alcoholic solutions with basic and acidic components.

A

Wright, Giemsa, or May-Grünwald stains

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21
Q

Counting Method

A
  1. cross sectional method
  2. longitudinal method
  3. battlement method
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22
Q

Examine the RBC morphology

A
  • Presence of inclusions and abnormalities
  • Presence of immature RBCs
  • Size and pallor of RBCs
  • Rouleaux and agglutination
  • Polychromatophilia
23
Q

Immature granulocytic cells

A

shift to the left

24
Q

Hypersegmented neutrophils

A

shift to the right

25
Q

is an iron containing protein molecule found in red blood cells which transport oxygen from the lungs to the body’s tissues.

A

Hemoglobin

26
Q

Hemoglobin A1

A

96-98%
It contains two alpha subunits and two beta subunits.

27
Q

Hemoglobin A2

A

1.5-3.2%
It contains two alpha subunits and two gamma subunits

28
Q

Hemoglobin F

A

0.5-0.8%
It contains two alpha subunits and two delta subunits.

29
Q

Decreased Hemoglobin

A

Anemia

30
Q

Increased Hemoglobin

A

Possible polycythemia

31
Q

Preferred for standard measurement of hemoglobin due to accuracy and precision of result.

A

Automated

32
Q

counter check the result released by the machine, or if there is no machine available in the area or laboratory.

A

Manual Testing

33
Q

Direct/Visual Colorimetry

A

ACID HEMATIN TECHNIQUE

34
Q

Hemoglobin is converted to Acid Hematin by mixing blood with

A

0.1 N Hydrochloric acid

35
Q

Photoelectric / Light Absorbance Method

A

CYANMETHEMOGLOBIN METHOD (HiCN)

36
Q

DRABKIN’S SOLUTION (pH 7.0 – 7.4)

A

Potassium cyanide 50 mg
Potassium ferricyanide 200 mg
Sodium bicarbonate 1 g
Distilled water 1 liter

37
Q

Blood is lysed with the use of

A

non-ionic detergents

38
Q

is the reference method for Hb estimation

A

cyanmethemoglobin method

39
Q

All Hb forms except ______ is estimated

A

sulphaemoglobin

40
Q

Another reason for turbidity is lipemic specimen. To correct this, add

A

0.1 ml of Patient’s plasma to 5ml of reagent and use the solution blank.

41
Q

are used to correct abnormal globulins.

A

Addition of potassium carbonate

42
Q

can be resistant to hemolysis and cause turbidity.

A

Hb S and Hb C

43
Q

NORMAL RANGES
Adult Males:
Adult Females:
Newborns and Infants:

A

13-17 g/dl
11.5-16.5 g/dl
14-22g/dl

44
Q

Carboxyhemoglobin =

A

hemoglobin + carbon monoxide

45
Q

hemoglobin in which ferrous iron are oxidize to ferric state.

A

Methemoglobin

46
Q

irreversible formation of hemoglobin from sulphonamides and aromatic amines.

A

Sulfhemoglobin

47
Q

is the volume of RBCs expressed as a percentage of the volume of whole blood in a sample

A

Hematocrit

48
Q

This test measures the proportion of the RBC to plasma in peripheral blood but not in the entire circulation

A

Hematocrit

49
Q

Decreased hematocrit

A

Anemia

50
Q

Increased hematocrit

A

Dehydration (Relative)
Polycythemia vera (Absolute)

51
Q

Not commonly used today since it is time consuming, requires larger amounts of blood, and generally contains a higher percentage of trapped plasma

A

MACROHEMATOCRIT METHOD

52
Q

with red band
Used for non-anticoagulated samples

A

Heparinized

53
Q

With blue band
Used for anticoagulated samples

A

Plain

54
Q

Capillary Tube Details

A

Approximately 75 mm long with
Approximately 1.2 mm inner bore

3rd year (26 decks)

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