CC LEC

Question 1

lamps for visible and near infrared

Answer 1

TUNGSTEN OR TUNGSTEN IODIDE

Question 2

lamps for visible and near infrared

Answer 2

TUNGSTEN OR TUNGSTEN IODIDE

Question 3

Incandescent light (nm)

Answer 3

400nm - 700nm

Question 4

lamps required for work in U.V. range

Answer 4

Deuterium or mercury-arc

Question 5

Range of mercury arc

Answer 5

160 - 375nm

Question 6

Purpose of Components Monochromator

Answer 6

▶ Promote spectral isolation
▶ Isolate a single wavelength of light
▶ Provides increased sensitivity & specificity

Question 7

Types of Components Monochromator

Answer 7

▶ Colored glass filters
▶ Prisms
▶ Diffraction gratings

Question 8

Measures the success of the monochromator

Answer 8

Bandpass/Bandwidth

Question 9

Defines the width of the segment of the spectrum that will be isolated by the monochromator

Answer 9

Bandpass/Bandwidth

Question 10

for work in the visible light range

Answer 10

Glass Cuvette

Question 11

for work in the UV range

Answer 11

Quartz or fused silica

Question 12

are cheaper but light refraction and distortion occur

Answer 12

Round cuvets

Question 13

have less light refraction but usually more costly

Answer 13

Square cuvette

Question 14

to convert the transmitted light into an
equivalent amount of electrical energy

Answer 14

Photodetectors

Question 15

to convert the electrical signal from the detector to a usable form

Answer 15

Read out device

Question 16

checked by using special filters with known peak transmission

Answer 16

wavelength accuracy

Question 17

verifies that the wavelength indicated on the dial is what is being passed through the monochromator.

Answer 17

wavelength accuracy

Question 18

▶ is any wavelength of light reaching the detector, outside the range of
wavelengths being transmitted by the monochromator.

Answer 18

stray light

Question 19

Causes insensitivity and linearity issues

Answer 19

stray light

Question 20

Made by reading the absorbance of a set of standard solutions (obtained commercially) at specified wavelength(s)

Answer 20

linearity

Question 21

photons of energy traveling in waves

Answer 21

Electromagnetic Radiation

Question 22

The energy of EMR is ____________ to wavelength.

Answer 22

inversely proportional

Question 23

The energy of EMR is ____________ to wavelength.

Answer 23

inversely proportional

Question 24

States the relationship between the absorption of light by a solution and the concentration of the material in the solution.

Answer 24

Beer’s law

Question 25

use a filter to produce light of one wavelength

Answer 25

Simple photometer/colorimeter

Question 26

Spectrophotometers differ from photometers in that they use ________ to form monochromatic light.

Answer 26

prisms or diffraction gratings

Question 27

Spectrophotometers differ from photometers in that they use prisms or diffraction gratings to form monochromatic light.

Answer 27

light source
monochromators
sample cell/cuvette
photodetector
display read-out device

Question 28

Components of simple photometer

Answer 28

Lamp
Filter
cuvet
photocell
meter

Question 29

based on the mathematical relationship between solute concentration & light absorbance

Answer 29

spectroscopy